“健康快车”特殊条件下眼科手术器械漂洗方法的研究  

Rinsing methods of ophthalmic surgical instruments under the special condition of“Health Express”

作　　者：马思文 孙怡雯 陈晨[1] 唐媛[1] 陈茹 陈燕燕 MA Si-wen;SUN Yi-wen;CHEN Chen;TANG Yuan;CHEN Ru;CHEN Yan-yan(School of Ophthalmology and Optometry, School of Biomedical Engineering, Wenzhou Medical University, Wenzhou 325000, China;Central Sterile Supply Department, Eye Hospital, Wenzhou Medical University, Wenzhou 325000, China;Nursing Department, Eye Hospital, Wenzhou Medical University, Wenzhou 325000, China)

机构地区：[1]温州医科大学眼视光学院生物医学工程学院,浙江温州325000 [2]温州医科大学附属眼视光医院消毒供应中心,浙江温州325000 [3]温州医科大学附属眼视光医院护理部,浙江温州325000

出　　处：《中国感染控制杂志》2022年第1期37-42,共6页Chinese Journal of Infection Control

基　　金：浙江省教育厅一般科研项目(Y202045483);温州市基础性科研资金资助项目(Y2020128);温州医科大学眼视光学院、附属眼视光医院、生物医学工程学院护理专项课题(YNHL1201901)。

摘　　要：目的探索在“健康快车”无流动水清洗设备的特殊条件下,复用眼科手术器械有效的漂洗方法。方法研究分两个阶段进行,分别探索不同漂洗方法对手术器械多酶清洗剂清除效果和清洗质量的影响。分别选择无污染和模拟临床手术污染的白内障显微手术器械。A、B、C、D、F、G组为试验组,模拟“健康快车”条件下使用非流动水漂洗手术器械,A、B、C 3组的漂洗方法分别为“2、4、6 L水结合刷洗”、D组“不漂洗”、F组漂洗方法同B组、G组在B组的基础上增加“0.5 L水结合涮洗”的步骤。E、H组为对照组,遵守指南使用流动水漂洗手术器械。清洗效果评价:采用蛋白残留检测仪间接检测多酶清洗剂残留量;采用ATP生物荧光检测仪检测手术器械的清洗质量。结果B组多酶清洗剂残留量为(1.90±0.23)μg/20把,B组与对照组[(3.45±0.46)μg/20把]的多酶清洗剂残留结果比较,差异无统计学意义(P=0.053)。G组ATP生物荧光检测结果为(9.46±1.50)RLU/把,G组ATP生物荧光检测结果与对照组[(11.60±1.44)RLU/把]比较,差异无统计学意义(P=0.339)。结论结合两阶段试验结果,在“健康快车”无流动水清洗设备的特殊条件下漂洗眼科手术器械,推荐选用G组漂洗方法。Objective To explore an effective rinsing method for reusing ophthalmic surgical instruments under the special condition of“Health Express”without running water cleaning equipment.Methods The study was divided into two stages to explore the effect of different rinsing methods on the removal effect and cleaning quality of multi-enzyme detergents for surgical instruments.The cataract microsurgical instruments without contamination and si-mulated clinical surgical contamination were selected respectively.Group A,B,C,D,F and G were trial groups,surgical instruments were rinsed with non-running water under the condition of simulated“Health Express”,rinsing methods of group A,B and C were“2 L/4 L/6 L water combined with brushing”,group D was with“no rinsing”,group F adopted the same rinsing method as group B,group G adding“0.5 L water combined with rinsing”on the basis of group B.Group E and H served as the control group,using running water to rinse surgical instruments in accordance with the guidelines.Evaluation of cleaning effect:protein residue detector was used to detect indirectly the residue of multi-enzyme detergent,and ATP bioluminescence detector was used to detect the cleaning quality of surgical instruments.Results The residual amount of multi-enzyme detergent in group B was(1.90±0.23)μg per 20 surgical instruments,difference between multi-enzyme detergent residue in group B and control group([3.45±0.46]μg per 20 surgical instruments)was not significant(P=0.053).ATP biofluorescence detection result in group G was(9.46±1.50)RLU per surgical instrument,difference between ATP biofluorescence detection result in group G and control group([11.60±1.44]RLU per instrument)was not significant(P=0.339).Conclusion Combined with the results of two-stage experiments,group G rinsing method is recommended for rinsing ophthalmic surgical instruments under the special condition of“Health Express”without running water cleaning equipment.

关 键 词：健康快车 清洗 漂洗 眼科 显微器械 白内障 

分 类 号：R187[医药卫生—流行病学]