一种新超敏酶促化学发光增强液制备研究  

Study of a New Hypersensitive Enzyme Enhanced Chemiluminescence Solution

作　　者：王静[1] 高雪 陈曦[1] 韩加如 Wang Jing;Gao Xue;Chen Xi;Han Jiaru(School of Pharmaceutical Engineering,Jiangsu Food&Pharmaceutical Science College,Huaian 223005,China)

机构地区：[1]江苏食品药品职业技术学院制药工程学院,江苏淮安223005

出　　处：《山东化工》2021年第24期1-4,10,共5页Shandong Chemical Industry

基　　金：淮安市自然科学研究计划(HAB201713);江苏省高等学校自然科学研究面上项目(19KJB350009);校级自然科学研究项目(JSSPMS2017203)。

摘　　要：以提高免疫诊断技术中信号检测的灵敏度为目的,开发新型超敏化学发光HRP型鲁米诺增强液。重点探讨鲁米诺、过氧尿素及不同的增强剂对荧光强度的影响,并采用Box-Behnken响应面优化法,最终形成增强液的最佳成分组合,并检测其性能。新型超敏化学发光液最佳成分为:鲁米诺2.5 mmol/L,3-(10-吩噻嗪)-丙酸钠6 mmol/L,4-二甲氨基吡啶4 mmol/L,过氧尿素13 mmol/L,在此条件下发光液效果较好,半衰期超过1 h,灵敏度为0.09 pg/孔,可于37℃存放1个月,低温长期保存。This study was aimed to improve the sensitivity of signal detection in immunodiagnostic techniques and to develop a new type of hypersensitive chemiluminescence HRP luminol reinforcing fluid.The effects of luminol,hydrogen peroxide and different enhancers on fluorescence intensity were discussed.Box-Behnken response surface methodology was conducted to optimize the design and experiment.The newly prepared chemiluminescent solution had better effect when luminol was 2.5 mmol/L,Sodium 3-(phenothiazin-10-yl)propionate was 6 mmol/L,4-morpholinopyridine was 4 mmol/L,and eroxide urea was 13 mmol/L.The half-life of decay of light emission is more than an hour.The assay sensitivity for HRP is 0.09 pg per well.It can be stored for 30 d at 37℃or for a long time at a low temperature.

关 键 词：辣根过氧化物酶 鲁米诺 化学发光 免疫印记 

分 类 号：TQ460[化学工程—制药化工] O643.32[理学—物理化学]