花生花斑种皮花青素生物合成相关miRNA及其靶基因鉴定与分析  

Identification and Analysis of miRNA and Its Target Genes Related to Anthocyanin Biosynthesis in Variegated Testa of Peanut

作　　者：胡梦蝶 李佳伟 王梅 刘彬 马钰聪 赵雨露 杨鑫雷[1] 崔顺立[1] 侯名语[1] 蒋晓霞 穆国俊[1] HU Meng-die;LI Jia-wei;WANG Mei;LIU Bin;MA Yu-cong;ZHAO Yu-lu;YANG Xin-lei;CUI Shun-li;HOU Ming-yu;JIANG Xiao-xia;MU Guo-jun(College of Agronomy,Hebei Agricultural University/National Key Laboratory of North China Crop Improvement and Regulation/Hebei Crop Germplasm Resources Laboratory,Baoding 071001;Hebei Yiyuan Ecological Agriculture Technology Co,Ltd,Baoding 071000)

机构地区：[1]河北农业大学农学院/华北作物种质资源研究与利用教育部重点实验室/河北省种质资源实验室,保定071001 [2]河北易园生态农业科技有限公司,保定071000

出　　处：《植物遗传资源学报》2022年第1期226-239,共14页Journal of Plant Genetic Resources

基　　金：河北省高等学校科学技术研究项目(ZD2019051);河北省重点研发计划项目现代种业科技专项(19226363D);易县易园农业科技园区建设(2111N004)。

摘　　要：花生在世界粮油食品中占有重要地位,其种皮颜色有白色、红色、紫色、粉红色及花斑等多种颜色,花斑种皮花生是其独特的成员之一,具有便于区分的优良性状。本研究以花斑种皮花生VG-02为研究材料,结果表明与花斑种皮颜色合成相关差异表达的miRNA富集的代谢途径有苯丙烷生物合成、类黄酮生物合成、异黄酮的生物合成、昼夜节律植物。miRNA测序结果中共筛选出86个差异表达miRNA,其中20个差异表达的miRNA与花生花斑种皮颜色合成相关。其中包括4个共同靶向花青素、类花青素和IFS2靶基因的miRNA,即miR_8、miR_50、miR_51和miR_239;5个靶向花青素生物合成中结构基因的miRNA,即调控CHS靶基因的miR_398-x,调控4CL靶基因的miR_482-z,调控F3′H靶基因的miR_266和miR_182以及调控花青素3-O-葡萄糖苷-6靶基因的miR_5;1个靶向花青素生物合成调节基因的miRNA,即miR858-y靶向调控MYB2和MYB3;10个靶向CYP450靶基因的miRNA,即miR_10、miR_15、miR_61、miR_72、miR_102、miR_116、miR_123、miR_193、miR_256和miR_862-z。miRNA测序与转录组联合分析KEGG代谢通路表明类黄酮生物合成为花生种皮花斑形成最直接的代谢途径。本研究对于全面揭示花斑花生种皮花青素合成的分子机制有重要意义。Peanuts are an important product as part of the world′s cereals,oils and foods.Peanut testa are white,red,purple,pink,and variegated.Variegated peanut testa is unique and can be distinguished from other types.In this study,the variegated testa peanut VG-02 was used as the research material.The study revealed that the differentially expressed miRNA-enriched metabolic pathways related to the color synthesis included phenylpropane biosynthesis,flavonoid biosynthesis,isoflavone biosynthesis and circadian rhythm-plant.Sequencing results showed that 86 miRNAs were differentially expressed,among which 20 miRNAs were related to the color synthesis in variegated testa peanut.Twenty differentially expressed miRNAs related to anthocyanin synthesis in variegated testa peanut included miR_8,miR_50,miR_51 and miR_239,which jointly targeted at anthocyanins,anthocyanidins and IFS2 target genes.Five miRNAs were found to target at structural genes in the anthocyanin biosynthesis:miR_398-x which regulates CHS target genes,miR_482-z which regulates 4CL target genes,miR_266 and miR_182 which regulate F3′H target genes,and miR_5 which regulates the anthocyanin 3-O-glucoside target gene.MiR858-y is a miRNA targeting at anthocyanin biosynthesis regulation gene,which targets at and regulates MYB2 and MYB3.MiR_10,miR_15,miR_61,miR_72,miR_102,miR_116,miR_123,miR_193,miR_256 and miR_862-z target at CYP450 target genes.The joined analysis of KEGG metabolic pathways by miRNA sequencing and transcriptome analysis indicated that flavonoid biosynthesis is the most direct metabolic pathway for the synthesis of testa variegation.This study furthers our understanding of the molecular mechanisms of anthocyanin synthesis in variegated testa peanut.

关 键 词：花生 花斑种皮 花青素生物合成 MIRNA 靶基因 

分 类 号：S565.2[农业科学—作物学]