呼吸链影响光化学反应过程中细胞外ATP调控作用的研究  

Study on the regulation of extracellular ATP in the process ofrespiratory chain affecting photochemical reaction

作　　者：冯汉青[1] 王建荷 张悦婧 李颖 庞海龙[1] FENG Han-qing;WANG Jian-he;ZHANG Yue-jing;LI Ying;PANG Hai-long(College of Life Sciences,Northwest Normal University,Lanzhou 730070,Gansu,China)

机构地区：[1]西北师范大学生命科学学院,甘肃兰州730070

出　　处：《西北师范大学学报（自然科学版）》2022年第1期77-83,共7页Journal of Northwest Normal University(Natural Science)

基　　金：国家自然科学基金资助项目(31870246,31560070);甘肃省高等学校科研项目(2015A-007);甘肃省重点研发计划资助项目(18YF1NA051);甘肃省高校基本科研业务费资助项目;西北师范大学青年创新团队项目。

摘　　要：细胞外ATP(eATP)是调节植物多种生理生化反应的信号分子.文中以野生型(WT)和eATP受体缺失突变(dorn-1)拟南芥(Arabidopsis thaliana)为实验材料,分析了呼吸链抑制剂水杨基氧肟酸(SHAM)影响植物光化学反应过程中eATP的调控作用.结果表明,SHAM能够显著降低交替呼吸途径的容量.在WT叶片中,SHAM处理能够引起光系统Ⅱ(PSⅡ)实际光化学效率(Y(Ⅱ))、相对电子传递速率(R_(ET))、光化学猝灭(q_(p))和PSⅡ处非调节性能量耗散的量子产量(Y(NO))的显著降低,同时使得非光化学猝灭(Q_(NP))和PSⅡ处调节性能量耗散的量子产量(Y(NPQ))显著上升;而在dorn-1叶片中,SHAM对Y(Ⅱ),R_(ET),qp,Q_(NP),Y(NPQ)和Y(NO)均无显著性影响.进一步研究发现,对WT叶片添加外源ATP可显著缓解SHAM所导致的Y(Ⅱ),R_(ET)和q_(p)的下降及Y(NPQ)的上升,而对DORN1叶片添加外源ATP则无类似效应.以上结果表明,eATP可通过受体蛋白DORN1对呼吸链抑制引起的光化学活性的下降进行调控.Extracellular ATP(eATP)as a signaling molecule regulates a variety of physiological and biochemical reactions of plants.Using wild-type(WT)and eATP receptor mutant(dorn-1)Arabidopsis thalianaas as test materials,this work analyzed the regulating role of eATP in the process of the photochemical reaction of plants in the presence of the respiratory chain inhibitor salicylhydroxamic acid(SHAM).The results showed that SHAM can significantly reduce the capacity of alternative respiratory pathways.In WT leaves,SHAM caused a significant decrease in Y(Ⅱ)(actual photosynthetic efficiency of PhotosystemⅡ),R_(ET)(relative electron transfer rate),q_(p)(photochemical quenching)and Y(NO)(quantum yield of unregulated energy dissipation at PSⅡ),and caused a significant increase in Q_(NP)(nonphotochemical quenching)and Y(NPQ)(the quantum yield of regulating energy dissipation at PSⅡ).However,in dorn-1 leaves,SHAM treatment did not significantly change the values of Y(Ⅱ),RET,qp,QNP,Y(NPQ)and Y(NO).Further experiments found that the addition of exogenous ATP can significantly alleviate the decrease in Y(Ⅱ),RET and qp,and the increase in Y(NPQ)of WT leaves caused by SHAM,while exogenous ATP failed to do so in the dorn-1 leaves.The above results indicated that eATP may regulate the decrease of photochemical activity caused by respiratory chain inhibition through the receptor protein DORN1.

关 键 词：拟南芥 DORN1 细胞外ATP 交替氧化酶途径 光系统Ⅱ 

分 类 号：Q942[生物学—植物学]