米卡芬净对光滑假丝酵母菌在巨噬细胞内活性的影响  

Effects of Micafungin on the Activity of Candida Smooth in Macrophages

作　　者：赵维佳 李红宾 陈宗翰 ZHAO Weijia;LI Hongbin;CHEN Zonghan(Dept.of Dermatology,The 1st Affiliated Hospital of Kunming Medical University,Kunming Yunnan,650032;Higher Education Research and Evaluation Center,Yunnan University of Chinese Medicine,Kunming Yunnan 650504,China)

机构地区：[1]昆明医科大第一附属医院皮肤科,云南昆明650032 [2]云南中医药大学高教评估中心,云南昆明650504

出　　处：《昆明医科大学学报》2022年第1期14-19,共6页Journal of Kunming Medical University

基　　金：云南省高层次卫生人才后备人才基金资助项目(H-2019034);云南省科技厅-昆明医科大学应用基础联合专项基金资助项目(202001AY070001-049);云南省教育厅科学研究基金资助项目(2018js211)。

摘　　要：目的探究米卡芬净对光滑假丝酵母菌在巨噬细胞内活性的影响。方法选取巨噬细胞为研究对象,分离自皮肤、阴道分泌物、脓液、血液和体腔液体标本,通过生化及分子生物学鉴定光滑假丝酵母菌株,以及1株标准菌株ATCC2001(购自国家菌种保藏中心),并经生物学鉴定,应用不同浓度的米卡芬净作用于光滑假丝酵母菌,分为对照组、造模组、低浓度组、中浓度组和高浓度组。测定不同时间SOD活性、MDA含量及NO含量,测定NF-Κb p65、BKca、NLRP3和Atg5蛋白表达水平,IL-6、IL-10、IL-1β、TNF-α水平。结果与对照组比较,造模组巨噬细胞刺激SOD活性显著降低,与造模组比较,低浓度组、中浓度组、高浓度组巨噬细胞刺激SOD活性水平显著增加,在5h达到最大值,其中低浓度组>中浓度组>高浓度组,差异有统计学意义(P<0.05);与对照组比较,造模组巨噬细胞分泌MDA及NO显著增加,与造模组比较,低浓度组、中浓度组、高浓度组细胞MDA及NO水平显著提高,其中分泌含量排序为:中浓度组>高浓度组>低浓度组,差异有统计学意义(P<0.05);与对照组比较,造模组巨噬细胞蛋白表达显著增加,与造模组比较,低浓度组、中浓度组、高浓度组巨噬细胞NF-Κb p65、BKca、NLRP3和Atg5蛋白表达显著提高,其中蛋白表达水平排序为:低浓度组>中浓度组>高浓度组,差异有统计学意义(P<0.05);与对照组比较,造模组巨噬细胞血清炎症因子水平显著增加;与造模组比较,低浓度组、中浓度组、高浓度组巨噬细胞IL-6、IL-10、IL-1β、TNF-α的水平显著提高,其中炎症因子的分泌排序为:低浓度组>中浓度组>高浓度组,差异有统计学意义(P<0.05)。结论米卡芬净有效刺激巨噬细胞的应激反应,调节免疫系统功能,抑制光滑假丝酵母菌的活性,起到较佳的抑菌效果。Objective To investigate the effects of micafungin on the activity of Candida glabra in macrophages.Methods Macrophages were selected as the research object,and samples were isolated from the skin,vaginal secretion,pus,blood and body cavity fluid.Candida smooth strain and a standard strain ATCC2001(purchased from the national strain collection center)were identified by biochemistry and molecular biology.Different concentrations of micafungin were used to treat Candida smooth.They were divided into the control group,model group,low concentration group,medium concentration group and high concentration group respectively.SOD activity,MDA content and no content were measured at the different times,and NF was measured-ΚB protein expression levels of p65,BKCa,NLRP3 and ATG5,IL-6,IL-10 and IL-1β,TNF-αLevel.Results Compared with the control group,the activity of SOD stimulated by macrophages in the modeling group was significantly decreased;Compared with the modeling group,the activity of SOD stimulated by macrophages in the low concentration group,medium concentration group and high concentration group was significantly increased,reaching the maximum value at 5 h,with the low concentration group>medium concentration group>high concentration group,and the difference was statistically significant(P<0.05).Compared with the control group,the secretion of MDA and NO by macrophages in the modeling group was significantly increased;Compared with the modeling group,the levels of MDA and NO in the low concentration group,medium concentration group and high concentration group were significantly increased,and the order of secretion content was as follows:medium concentration group>high concentration group>low concentration group,the difference was statistically significant(P<0.05).The protein expressions of NF-κB P65,BKca,NLRP3 and Atg5 were significantly increased in the modeling group compared with the control group,and the protein expressions of NF-κB P65,BKca,NLRP3 and Atg5 were significantly increased in the low-concent

关 键 词：米卡芬净 光滑假丝酵母菌 巨噬细胞 蛋白表达 

分 类 号：R519[医药卫生—内科学]