GDM孕妇网膜脂肪组织中Chemerin的表达与IRS-1及其酪氨酸磷酸化分析  

Analysis of Chemerin Expression and IRS-1 and Tyrosine Phosphorylation in Omental Adipose Tissue of GDM Pregnant Women

作　　者：李晓红[1] 赵琳[1] 张馨文[1] 陈卓[1] 马润玫[1] LI Xiaohong;ZHAO Lin;ZHANG Xinwen;CHEN Zhuo;MA Runmei(Dept.of Obstetrics and Gynecology,The 1st Affiliated Hospital of Kunming Medical University,Kunming Yunnan 650032,China)

机构地区：[1]昆明医科大学第一附属医院产科,云南昆明650032

出　　处：《昆明医科大学学报》2022年第1期48-52,共5页Journal of Kunming Medical University

基　　金：国家自然科学基金项目资助项目(81160082);云南省科技厅-昆明医科大学应用基础联合专项基金资助项目(202001AC070082);博士科研启动基金资助项目(2016BS004)。

摘　　要：目的通过检测网膜脂肪组织中Chemerin的表达与胰岛素受体底物-1表达及酪氨酸磷酸化水平,探讨Chemerin与GDM胰岛素抵抗的关系。方法将研究对象分为GDM组及正常对照组NGT各22名,剖宫产术中收集网膜脂肪组织2g,Western blot及qPCR方法检测胎盘组织中Chemerin、IRS-1的表达及免疫沉淀法检测IRS-1的酪氨酸磷酸化水平。结果GDM组网膜脂肪组织中Chemerin蛋白质表达显著增加[2.99(0.04,7.90)vs 2.21(0.02,13.30),P=0.010];GDM组IRS-1[3.52(2.51,4.49)vs 5.47(4.04,8.98),P=0.024]蛋白表达明显降低;GDM组IRS-1酪氨酸磷酸化[0.60(0.01,1.74)vs 4.06(0.06,54.41),P=0.012]显著降低;相关性分析发现Chemerin蛋白表达与OGTT2h血糖密切相关;Chemerin mRNA与产前BMI密切相关;Chemerin蛋白表达与IRS-1蛋白正相关;IRS-1酪氨酸磷酸化水平与OGTT 0 h PG、OGTT 2 h PG密切负相关。结论网膜脂肪组织中Chemerin表达与IRS-1信号异常密切相关是其引起GDM胰岛素抵抗因素之一。Objective To investigate the relationship between chemerin and insulin resistance in GDM by detecting the expression of insulin receptor substrate-1 and tyrosine phosphorylation in omental adipose tissue.Methods Subjects were divided into the GDM group 24 and the normal control group with 22 NGT.Two grams of omental adipocytes were collected during the cesarean section.The expression of chemerin and IRS-1 in placental tissues was detected with Western blot and qPCR,and the tyrosine phosphorylation of IRS-1 was detected with the immunoprecipitation.Results The expression of chemerin protein in retinal adipocytes in GDM group was significantly increased(2.99(0.04,7.90)vs 2.21(0.02,13.30),P=0.010).The protein expression of IRS-1(3.52(2.51,4.49)vs 5.47(4.04,8.98),P=0.024)was significantly decreased in GDM group.The phosphorylation of IRS-1 tyrosine was significantly decreased in GDM group(0.60(0.01,1.74)vs 4.06(0.06,54.41),P=0.012).Correlation analysis showed that chemerin protein expression was closely related to OGTT2 h glucose.Chemerin mRNA was closely correlated with prenatal BMI.Chemerin protein expression was positively correlated with IRS-1 protein.IRS-1 tyrosine phosphorylation was negatively correlated with OGTT 0 h PG and OGTT 2 h PG.Conclusion The expression of chemerin in omental adipose tissue is closely related to IRS-1 signaling,which is one of the factors causing insulin resistance in GDM.

关 键 词：GDM CHEMERIN IRS-1 酪氨酸磷酸化 胰岛素抵抗 

分 类 号：R714.2[医药卫生—妇产科学]