基于UPLC-LTQ-Orbitrap-MS技术分析黄芩清肺汤中黄芩、栀子单煎与合煎的成分变化  

The Composition Changes of Mixed-decoction and Co-decoction of Scutellariae Radix and Gardeniae Fructus in Huangqin Qingfei Decoction Based on UPLC-LTQ-Orbitrap-MS

作　　者：梁琨[1] 张倩芸 沈莉玲 安叡[1] 王新宏[1] 尤丽莎[1] LIANG Kun;ZHANG Qianyun;SHEN Liling;AN Rui;WANG Xinhong;YOU Lisha(Shanghai University of Traditional Chinese Medicine,Shanghai 201203,China)

机构地区：[1]上海中医药大学,上海201203

出　　处：《中药新药与临床药理》2021年第12期1859-1864,共6页Traditional Chinese Drug Research and Clinical Pharmacology

基　　金：上海中医药大学预算内项目(A1-GY20-102-0804)。

摘　　要：目的研究黄芩清肺汤中黄芩、栀子配伍前后的成分变化,探讨二者配伍的合理性。方法采用超高效液相色谱-离子阱-静电场轨道阱质谱联用(UPLC-LTQ-Orbitrap-MS)技术建立黄芩、栀子配伍前后的化学指纹图谱,通过正交偏最小二乘判别法分析药对配伍前后的化学成分变化,并对筛选到的化学标记物进行鉴定。结果黄芩、栀子合并液与合煎液化学成分存在明显差异,合煎后京尼平苷酸(geniposidic acid)、栀子苷(gardenoside)等含量显著下降,黄芩素(baicalein)、槲皮素(quercetin)等含量显著升高。结论黄芩清肺汤中黄芩、栀子配伍后,可促进多种活性成分在合煎液中的溶出,这些成分可能是黄芩、栀子配伍协同增效的物质基础。Objective To investigate the composition changes of Scutellariae radix and Gardeniae fructus in Huangqin Qingfei Decoction before and after compatibility,and to clarify the rationality of compatibility.Methods UPLC-LTQ-Orbitrap-MS was used to establish the chemical fingerprint spectra before and after co-decoction.The changes of chemical components were analyzed by orthogonal partial least squares discriminant analysis,and the selected chemical markers were identified.Results There were significant differences in contents of compositions between the mixed-decoction and the co-decoction.The contents of geniposidic acid and gardenoside decreased significantly,while the contents of bacalein and quercetin increased significantly after compatibility.Conclusion The combination of Scutellariae radix and Gardeniae fructus in Huangqin Qingfei Decoction can promote the dissolution of various active components in the co-decoction,which may be the material basis of synergistic effect of Scutellariae radix and Gardeniae fructus.

关 键 词：黄芩清肺汤 黄芩 栀子 配伍 协同增效 超高效液相色谱-离子阱-静电场轨道阱质谱联用 

分 类 号：R284.1[医药卫生—中药学]