茶麸黄酮促进毛乳头细胞增殖和VEGF分泌的研究  

Study on the promotion of proliferation of dermal papilla cells and VEGF secretion by flavonoids from camellia seed cake

作　　者：沈胡驰 陈殿松 杨井国 王靖 Huchi Shen;Diansong Chen;Jingguo Yang;Jing Wang(Cosmetic Research Center,School of Chemistry and Material Engineering,Jiangnan University,Wuxi,Jiangsu 214122,China;Guangzhou Degu Personal Care Products Co.,Ltd.,Adolf Research Innovation Laboratory,Guangzhou,Guangdong 510000,China)

机构地区：[1]江南大学化学与材料工程学院化妆品研究中心,江苏无锡214122 [2]阿道夫科研创新实验室广州德谷个人护理用品有限公司,广东广州510000

出　　处：《日用化学工业》2022年第1期44-49,共6页China Surfactant Detergent & Cosmetics

基　　金：国家自然科学基金资助项目(51802123)。

摘　　要：以脱脂茶麸为原料,通过乙醇水溶液超声波辅助提取脱脂茶麸中的黄酮,探究其生物活性。用不同质量浓度含茶麸黄酮溶液作用于体外培养的人毛乳头细胞(HDPCs),四甲基偶氮唑蓝(MTT)法测定细胞活性,并通过流式细胞术检测茶麸黄酮对细胞凋亡、周期的影响以及ELISA法检测细胞培养上清液中血管内皮生长因子(VEGF)的变化。结果表明,茶麸黄酮富集产物对HDPCs具有增殖作用,质量浓度20μg/mL时增殖效果最佳,比10μg/mL米诺地尔阳性对照效果显著(**P<0.01)。茶麸中黄酮可能是通过减少HDPCs的早凋,促进HDPCs的有丝分裂从而对细胞的增殖发生作用。茶麸黄酮富集产物对HDPCs的VEGF分泌呈现浓度依赖性。Defatted camellia seed cake was used as raw material,and the flavonoids therein were extracted with aqueous ethanol solution with the aid of ultrasonic wave to explore their biological activity.Different mass concentrations of camellia seed cake flavonoids were applied to human dermal papilla cells (HDPCs) cultured;in vitro,and the cell viability was determined by MTT method.The effects on apoptosis and cell cycle were detected by flow cytometry.ELISA method was used to detect the change of vascular endothelial growth factor (VEGF);in cell culture supernatant.The results showed that flavonoid-enriched product of camellia seed cake had a proliferation effect on HDPCs and could promote the secretion of VEGF in a concentration-dependent manner.The proliferation effect was the best when the concentration of flavonoid-enriched product was 20μg/m L,which was more effective than the positive control of10μg/m L minoxidil (;P<0.01).The flavonoids in camellia seed cake might reduce the premature apoptosis of HDPCs and promote the mitosis of HDPCs and thus lead to an effect on their proliferation.

关 键 词：茶麸 黄酮 毛乳头细胞 细胞周期 血管内皮生长因子 

分 类 号：TQ658[化学工程—精细化工]