艾灸对肠易激综合征模型大鼠IL-1β、IL-10和CGRP表达的影响  

Influence of Moxibustion on IL-1β,IL-10 and CGRP expression in irritable bowel syndrome model rats

作　　者：史先芳 尚精娟[2] 徐晖[2] SHI Xian-fang;SHANG Jing-juan;XU Hui(Department of Gastroenterology,Qingpu Branch of Zhongshan Hospital Affiliated Fudan Universityy Shanghai 201700,China;Department of Gastroenterology,The Seventh People's Hospital of Shanghai University of Traditional Chinese Medicine,Shanghai 200137,China)

机构地区：[1]复旦大学附属中山医院青浦分院消化科,上海201700 [2]上海中医药大学附属第七人民医院消化科,200137

出　　处：《哈尔滨医科大学学报》2021年第5期462-465,共4页Journal of Harbin Medical University

基　　金：国家自然科学基金青年基金资助项目(81503541)。

摘　　要：目的探讨艾灸对肠易激综合征(irritable bowel syndrome,IBS)模型大鼠IL-1β、IL-10和CGRP表达的影响。方法建立IBS模型,将大鼠随机分为实验1组、实验2组、实验3组、实验4组。测定结肠转运功能、小肠推进率、Elisa法检测血浆IL-1β、IL-10和CGRP的表达。结果与实验1组比较,实验2组、实验3组、实验4组大鼠的玻璃小球排出时间显著减少(P<0.01)、小肠推进率显著增加(P<0.01);与实验2组比较,实验3组、实验4组的排出时间显著增加(P<0.01)、小肠推进率显著减少(P<0.01);实验3组和实验4组比较无统计学意义(P>0.05)。与实验1组比较,IL-1β和CGRP的表达在实验2组显著上升,IL-10的表达下降(P<0.01);与实验2组比较,实验3组和实验4组的IL-1β和CGRP的表达水平显著降低,而IL-10的表达显著上升(P<0.01)。结论艾灸通过调节炎症因子IL-1β、IL-10和CGRP的表达,降低小肠推进率,从而改善肠易激综合征患者的症状。Objective To study the effect of Moxibustion on IL-1β,IL-10 and CGRP expression in irritable bowel syndrome rats.Methods IBS model was established and the rats were randomly divided into experimental group 1,experimental group 2,experimental group 3 and experimental group 4.The colonic transport function and small intestine propulsion rate were detected,and the expression of plasma IL-1β,IL-10 and CGRP by Elisa method were detected.Results Compared with the experimental group 1,the time of glass pellet excretion in the experimental group 2,experimental group 3,and experimental group 4 was significantly shortened(P<0.01),and the small intestine propulsive rate was obviously elevated(P<0.01);Compared with the experimental group 2,the excretion time of experimental group 3 and experimental group 4 was significantly prolonged(P<0.01),and the small intestine advancement rate was significantly reduced(P<0.01);There was no statistical significance between experimental group 3 and experimental group 4(P>0.05).Compared with experimental group 1,the expression of IL-1βand CGRP increased significantly in the experimental group 2,and the expression of IL-10 decreased(P<0.01);Compared with the experimental group 2,the expression of IL-1βand CGRP in the experimental group 3 and experimental group 4 were significantly reduced,while the expression of IL-10 was significantly increased(P<0.01).Conclusion Moxibustion can decrease the rate of small intestine propulsion and improve the symptoms in patients with irritable bowel syndrome by regulating the expression of inflammatory factors IL-1β,IL-10 and CGRP.

关 键 词：艾灸 IL-1Β IL-10 CGRP 

分 类 号：R574[医药卫生—消化系统] R245.81[医药卫生—内科学]