纳米碳对百合试管苗生长代谢及生理特性的影响  

Effect of Nano-carbon on the Growth Metabolism and Physiological Characteristics of Lily Test-tube Plantlet

作　　者：张芳[1] 任建宏[2] 王育选[2] 赵娟[2] ZHANG Fang;REN Jian-hong;WANG Yu-xuan;ZHAO Juan(College of Information/Shanxi Agricultural University,Taigu 030801,China;College of Agricultural/Shanxi Agricultural University,Taigu 030801,China)

机构地区：[1]山西农业大学信息学院,山西太谷030801 [2]山西农业大学农学院,山西太谷030801

出　　处：《山东农业大学学报（自然科学版）》2021年第6期911-915,共5页Journal of Shandong Agricultural University：Natural Science Edition

基　　金：山西省面上青年基金项目(201601D202054);山西省科技攻关项目(20130311014-1);山西农业大学博士科研启动项目(2012YJ13)。

摘　　要：为研究纳米碳对百合试管苗生长代谢及生理特性的影响,在优化百合试管苗培养基的同时,探讨纳米碳在离体培养中的作用,为其应用于植物组织培养提供理论参考。本文以平陆百合脱毒试管苗为外植体,接种于附加不同浓度纳米碳的培养基中,了解纳米碳对百合鳞茎膨大、生根、增殖等生长情况的影响,并测定其干重、叶绿素含量、根系活力、全氮含量等生理指标,分析纳米碳对百合试管苗生理代谢的影响。结果表明:纳米碳对百合试管苗鳞茎膨大、增殖、生根、物质积累、根系活力、抗氧化性等均表现不同程度的促进作用。百合试管苗膨大、增殖倍率、生根数、地上部和地下部干重、全氮、还原糖、Vc含量、根系活力、叶绿素含量、SOD、POD、CAT活性基本随纳米碳浓度提高先升高后降低,MDA含量则先降低后升高,这些指标达到峰值时所需的纳米碳浓度不同。综合分析,纳米碳适宜的附加浓度为400~600 mg·L^(-1),可调节百合试管苗生理生化过程,明显促进其生长。In order to study the effect of nano-carbon on the growth and metabolism and physiological characteristics of lily tube seedling, the role of nano-carbon in vitro culture was studied while optimizing the culture medium of lily tube seedling so as to provide theoretical reference for its application in plant tissue culture. In this paper, the virus-free plantlets of Lilium pinglu were used as explants and inoculated in the medium supplemented with different concentrations of nano carbon. Thus the effects of nano carbon on lily bulb expansion, rooting and proliferation were investigated as well as the physiological indexes such as dry weight, Chlorophyll content, root activity and total nitrogen content were measured to analyze the influence of nano carbon on the physiological metabolism of Lily tube seedlings. The results showed that the nano-carbon had different degree of promoting effect on the bulbous expansion, its proliferation, root activity, rooting matter accumulation,antioxidant activity of Lily test-tube plantlet. Lily tube seedlings inflated, proliferation rate, rooting number, above-ground dry weight as well as underground dry weight, total nitrogen, reducing sugar, Vc content, root activity, Chlorophyll content,SOD, POD, CAT activity basically increased first and then decreased with the increase of nano carbon concentration while MDA content decreased first and then increased instead. And the concentration of nanocarbon required would be different when these indexes reach to peak. According to the comprehensive analysis, the appropriate additional concentration of carbon nanoparticles was 400~600 mg·L^(-1), which could regulate the physiological and biochemical process of lilium tube-seedling and significantly promote its growth.

关 键 词：纳米碳 百合 试管苗 生长代谢 生理特性 

分 类 号：S644.1[农业科学—蔬菜学]