草莓种子实生苗病毒分子检测  

Molecular Detection of Virus in Strawberry Seed Seedlings

作　　者：缪军[1] 马秀明 孙杨[1] 兰成云 王俊峰[1] 韩伟[1] MIAO Jun;MA Xiuming;SUN Yang;LAN Chengyun;WANG Junfeng;HAN Wei(Institute of Vegetables,Shandong Academy of Agricultural Sciences/Shandong Key Laboratory of Greenhouse Vegetable Biology/Shandong Branch of National Vegetable Improvement Center,Ji′nan Shandong 250100)

机构地区：[1]山东省农业科学院蔬菜研究所/山东省设施蔬菜生物学重点实验室/国家蔬菜改良中心山东分中心,山东济南250100

出　　处：《现代农业科技》2022年第2期41-44,50,共5页Modern Agricultural Science and Technology

基　　金：山东省农业科学院农业科技创新工程项目(CXGC2018E08)。

摘　　要：草莓易感染病毒,导致产量减少和品质下降。目前生产上主要采用茎尖组织培养脱毒的方法,并不能保证完全脱除病毒,还需要进行无病毒检测鉴定。因此,需进一步探索简便、高效、免检的繁育体系。研究表明,种子繁育可以避免草莓镶脉病毒、草莓斑驳病毒、草莓轻型黄边病毒、草莓皱缩病毒的传播。本文利用RT-PCR技术,以草莓肌动蛋白基因序列为内标,结合4种草莓病毒特异性扩增,验证了草莓种子实生苗确定不携带病毒,以助推种子繁殖型草莓品种的应用。Strawberry can be infected by virus easily,which leads to yield and quality losses.At present,the method of stem tip tissue culture and detoxification is mainly used in production,but it can not guarantee the complete removal of the virus,virus-free detection and identification is needed.Therefore,it is necessary to further explore a simple,efficient and free-test propagation system.Studies have shown that seed reproduction can avoid the spread of strawberry vein banding virus,strawberry mottle virus,strawberry mild yellow edge virus and strawberry crinkle virus.Using RT-PCR technology,taking strawberry actin gene sequence as internal standard,combined with specific amplification of four strawberry viruses,this paper verified that strawberry seed seedlings did not carry the virus,so as to promote the application of seed reproductive strawberry varieties.

关 键 词：草莓 种子实生苗 病毒 分子检测 

分 类 号：S668.4[农业科学—果树学]