尿素对蛋白激发子AMEP功能活性的影响  

Effect of Urea on Functional Activity of Protein Elicitor AMEP

作　　者：万恒东 岳文涛 赵锋 商宪富 陈道文 王思文 孙明超 刘权[1] WAN Hengdong;YUE Wentao;ZHAO Feng;SHANG Xianfu;CHEN Daowen;WANG Siwen;SUN Mingchao;LIU Quan(Heilongjiang Bayi Agricultural University,Daqing Heilongjiang 163319;Hongwuyue Farm,Jiusan Branch,Beidahuang Group,Nenjiang Heilongjiang 161400)

机构地区：[1]黑龙江八一农垦大学,黑龙江大庆163319 [2]北大荒集团九三分公司红五月农场,黑龙江嫩江161400

出　　处：《现代农业科技》2022年第2期97-99,107,共4页Modern Agricultural Science and Technology

基　　金：2021年全国基层农技推广体系改革与建设补助项目“AMEP蛋白免疫激活剂在九三大豆的绿色增产示范”(2021-93157)。

摘　　要：为了探究尿素对蛋白激发子AMEP功能活性的影响,设置了一系列浓度梯度的尿素对AMEP蛋白进行处理,通过烟草叶片的过敏反应来检测尿素对AMEP的功能活性影响。结果表明,低浓度尿素不会影响AMEP蛋白的功能活性,而尿素浓度超过0.5 mg/mL,AMEP蛋白的功能活性则受到明显抑制。灰霉菌发病试验结果表明,0.05 mg/mL尿素处理的AMEP蛋白能够提高植物的抗病性。综上所述,AMEP蛋白可与0.05 mg/mL尿素配伍使用,为尿素与AMEP蛋白的共同使用提供了理论依据。In order to explore the effect of urea on the functional activity of protein elicitor AMEP,a series of con-centration gradient urea was set to treat AMEP protein,and the effect of urea on the functional activity of AMEP protein was detected by the hypersensitive reaction of tobacco leaves.The results showed that low concentration urea had no impact on the functional activity of AMEP protein.When the concentration of urea was more than 0.5 mg/mL,the functional activity of AMEP protein was significantly inhibited.Results of Botrytis cinerea incidence experiment showed that AMEP protein treated with 0.05 mg/mL urea could improve the disease resistance of plants.To sum up,AMEP protein could be applied with 0.05 mg/mL urea,which provided a theoretical basis for the joint application of urea and AMEP.

关 键 词：AMEP 尿素 枯草芽孢杆菌 叶面肥 灰霉菌 

分 类 号：TQ458[化学工程—农药化工]