高良姜查尔酮合成酶基因的生物信息学和表达分析  

Bioinformatics and Expression Analysis of Gene Encoding Chalcone Synthase from Alpinia officinarum

作　　者：黄琼林 HUANG Qionglin(Guangdong Medical University,Zhanjiang 524023 Guangdong,China)

机构地区：[1]广东医科大学,广东湛江524023

出　　处：《中药新药与临床药理》2021年第11期1726-1730,共5页Traditional Chinese Drug Research and Clinical Pharmacology

基　　金：广东省中医药局中医药科研项目(20211224)。

摘　　要：目的获取高良姜查尔酮合成酶(chalcone synthase,CHS)编码基因cDNA序列,明确其序列和功能特征以及在高良姜中的表达模式。方法在前期高良姜转录组测序数据中挖掘CHS的cDNA全长及其编码区,利用生物信息学方法对该基因蛋白的特征和功能进行分析,运用ClustalX1.83和MEGA4.0软件进行同源性比对和系统进化树构建,采用实时荧光定量PCR(q PCR)分析CHS在高良姜不同组织中的表达差异。结果高良姜CHS编码区为1 173 bp,编码391个氨基酸。其编码蛋白是分子量为94.5 k Da的亲水性蛋白,不含有信号肽、转运肽、靶肽和跨膜结构域,具有CHS保守功能域并归属于CHS超级家族。CHS在高良姜不同组织中的表达模式为:根茎>茎>叶。结论成功获得高良姜CHS序列,并分析其特征和表达模式,可为高良姜黄酮类有效成分的生物合成研究提供参考。Objective To obtain cDNA sequence of the gene encoding chalcone synthase(CHS),and clarify its sequence,function characteristics,and expression pattern in Alpinia officinarum.Methods The sequence of CHS gene was identified in the established transcriptome databases,and then the feature and function of coding protein was analyzed using bioinformatics approaches.The sequence alignment and polygenetic tree construction were performed by ClustalX1.83 and MEGA4.0 software.The expression difference of CHS gene in various tissues of A.officinarum was accomplished using qPCR.Results The length of CHS gene was 1 173 bp,which encoded391 amino acids.The CHS protein with a molecular weight of 94.5 k Da was hydrophilic,possessed none signal peptide,transit peptide,target peptide and transmembrane domain,and contained the functional domains defined as chalcone synthase,and was categorized into chalcone synthase superfamily.The CHS gene expressed the highest in rhizome,followed by stems,and the least in leaves of A.officinarum.Conclusion A CHS gene was successfully cloned from A.officinarum,and its sequence feature and expression pattern were also discussed,which provided a reference for the biosynthesis of the medicinal flavones in A.officinarum.

关 键 词：高良姜 查尔酮合成酶 生物信息学 表达模式 

分 类 号：R282.1[医药卫生—中药学]