大口黑鲈苗种致病性类志贺邻单胞菌的分离和鉴定  

Isolation and identification of pathogenic Plesiomonas shigelloides isolated from largemouth bass(Micropterus salmoides)

作　　者：庆辉 周国勤 陆健 张佳佳 王佩佩 QING Hui;ZHOU guoqin;LU Jian;ZHANG Jiajia;WANG Peipei(Institute of Fisheries Sciences of Nanjing City,Nanjing 210036,China)

机构地区：[1]南京市水产科学研究所,江苏南京210036

出　　处：《水产科技情报》2022年第1期43-47,共5页Fisheries Science & Technology Information

基　　金：江苏省政策引导类计划(苏北科技专项)“智能化加州鲈繁育系统研究与示范”(SZ-SQ2020023);2021年南京市公益性服务体系项目“特色淡水鱼引育种中心”。

摘　　要：对江苏南京某养殖场温室养殖的患病大口黑鲈鱼种进行病原分离,得到1株优势菌株,命名为Q-1。对其进行生化检测以及回归感染试验,结合16S rRNA基因序列测定,确定菌株Q-1为类志贺邻单胞菌(Plesiomonas shigelloides)。药敏试验结果表明,菌株Q-1对恩诺沙星、硫酸新霉素、氟苯尼考、盐酸多西环素和氟甲喹敏感,对甲砜霉素、磺胺间甲氧嘧啶钠和磺胺甲恶唑+甲氧苄啶具有耐药性。A dominant strain,named Q-1,was isolated from larvae of largemouth bass(Micropterus salmoides)in a greenhouse in Nanjing,Jiangsu.The pathogen of Q-1 was identified as Plesiomonas shigelloides by biochemical detection,regression infection test and 16S rRNA gene sequencing.The results of drug sensitivity test showed that the strain Q-1 was sensitive to enrofloxacin,neomycin sulfate,florfenicol,doxycycline hydrochloride and flumequine,and resistant to thiamphenicol,sulfamonomethoxine sodium and sulfamethoxazole+trimethoprim.

关 键 词：大口黑鲈 类志贺邻单胞菌 药敏试验 16S rRNA 

分 类 号：S85[农业科学—兽医学]