ETV5基因敲除对小鼠肌肉基因表达谱的影响  

Effect of ETV5 knockout on gene expression profile in mouse muscle

作　　者：万伟粲 张仙玉 赵鑫[1] 李彬[1] 蔡更元[1] 杨化强[1] 徐铮[1] WAN Weican;ZHANG Xianyu;ZHAO Xin;LI Bin;CAI Gengyuan;YANG Huaqiang;XU Zheng(College of Animal Science,South China Agricultural University,Guangzhou 510642,China)

机构地区：[1]华南农业大学动物科学学院,广东广州510642

出　　处：《华南农业大学学报》2022年第2期26-33,共8页Journal of South China Agricultural University

基　　金：广东省基础与应用基础研究基金项目(2019B1515210030)。

摘　　要：【目的】与野生型小鼠相比较,采用CRISPR/Cas9技术制备的ETV5基因纯合敲除小鼠在表现出内源性精原干细胞消融的同时伴随着体型和体质的明显弱势,本研究的目的是探究ETV5基因敲除对小鼠肌肉表达谱的影响。【方法】采集6周龄的3只野生型公鼠和3只ETV5纯合敲除公鼠的肌肉组织并抽提RNA,进行转录组测序,并对测序结果进行生物信息学分析,对2组小鼠肌肉样品的差异表达基因进行聚类分析、GO和KEGG富集分析。【结果】2组小鼠的肌肉组织共筛选出了574个差异表达基因,其中,上调基因292个,下调基因282个。发现了多个基因影响ETV5敲除小鼠的生长发育,其中,包括影响小鼠肌肉发育的基因Amd1和影响脂肪累积的基因Chrna2。GO和KEGG分析富集到的通路大多与脂肪代谢和生长发育相关。【结论】本研究结果为ETV5敲除小鼠生长发育缓慢和延迟的分子机制提供了解释,为研究ETV5在生理和病理上的相关功能提供了参考。【Objective】Compared with the wild type mice,the homozygous ETV5 knockout mice prepared by CRISPR/Cas9 technology showed significant weakness in body size and body weight along with endogenous spermatogonial stem cell ablation.The purpose of this study was to explore the effect of ETV5 knockout on the muscle expression profile of mice.【Method】The muscle tissues of three wild-type male mice and three ETV5 homozygous knockout male mice aged six weeks were collected and total RNA was extracted for transcriptome sequencing.We analyzed the sequencing results using bioinformatic method.Cluster analysis,GO and KEGG enrichment analyses were performed on the differentially expressed genes in the muscle samples of two groups of mice.【Result】A total of 574 differentially expressed genes were screened out from the muscle tissues of two groups,including 292 up-regulated genes and 282 down-regulated genes.Several genes were found to affect the growth and development of ETV5 knockout mice.These genes included Amd1 affecting muscle development of mice,and Chrna2 affecting fat accumulation.Most of the pathways enriched by GO and KEGG analyses were related to fat metabolism and growth and development.【Conclusion】These results provide an explanation for the molecular mechanism of abnormal development of ETV5 knockout mice,and provide references for further study of in vivo function of ETV5 gene.

关 键 词：ETV5 转录组测序 小鼠 肌肉发育 基因敲除 基因表达谱 

分 类 号：S813.3[农业科学—畜牧学]