高效液相色谱法检测5种市售鸡蛋中牛磺酸含量  

Determination of Taurine Content in Five Brands of Commercially Available Eggs by High Performance Liquid Chromatography

作　　者：秦梦然 张伟 吴凤明 刘付 蔡锋隆 白敬 王桐 于晓雪 李留安 QIN Meng-ran;ZHANG Wei;WU Feng-ming;LIU Fu;CAI Feng-long;BAI Jing;WANG Tong;YU Xiao-xue;LI Liu-an(Tianjin Key Laboratory of Agricultural Animal Breeding and Healthy Husbandry,College of Animal Science and Veterinary Medicine,Tianjin Agricultural University,Tianjin 300392,China;Tianjin Guangyuan Livestock and Poultry Breeding Co.,Ltd.,Tianjin 301806,China)

机构地区：[1]天津农学院动物科学与动物医学学院天津市农业动物繁育与健康养殖重点实验室,天津300392 [2]天津市广源畜禽养殖有限公司,天津301806

出　　处：《食品研究与开发》2022年第1期167-172,共6页Food Research and Development

基　　金：国家自然科学基金项目(31702223);天津市企业科技特派员项目(19JCTPJC59500);天津市科技重大专项与工程项目(18ZXBFNC00310);天津市“131”创新型人才团队建设项目(20180318)。

摘　　要：建立高效液相色谱技术检测鸡蛋中牛磺酸含量的方法,并对市售A、B品牌乌鸡蛋,C、D品牌柴鸡蛋以及普通鸡蛋蛋黄中的牛磺酸含量进行分析比较。试验采用邻苯二甲醛(ortho-phthalaldehyde,OPA)为衍生剂,利用自动进样器的在线衍生功能进行柱前衍生,流动相为甲醇+乙腈∶水(25∶75,体积比),荧光法定量。结果表明:使用该方法,牛磺酸在1μg/mL~25μg/mL具有良好的线性关系(R2=0.999 9),检测限为0.01μg/mL,回收率范围为99.7%~100.5%,相对标准偏差(relative standard deviation,RSD)为0.5%~0.6%;用该方法测定A、B品牌乌鸡蛋,C、D品牌柴鸡蛋和普通鸡蛋的蛋黄,牛磺酸含量分别为1.332、1.151、1.063、0.735、0.622 mg/100 g,普通鸡蛋蛋黄中的牛磺酸含量显著低于A、B品牌乌鸡蛋和C品牌柴鸡蛋(P<0.05),略低于D品牌柴鸡蛋(P>0.05)。该方法简单易行,回收率高,精密度高,特异性强,适用于测定鸡蛋中牛磺酸含量。A method for the determination of taurine content in eggs by high performance liquid chromatography(HPLC)was established,and the taurine contents in egg yolks of dark eggs(brands A and B),native eggs(brands C and D),and ordinary eggs was analyzed and compared. In the experiment,ortho-phthalaldehyde was used as the derivating agent,and the on-line derivatization function of the auto sampler was used for precolumn derivatization. A methanol/acetonitrile:water(25∶75,volume ratio)mobile phase and fluorescence detection were used. The results demonstrated good linearity in the concentration range of 1 μg/mL to 25 μg/mL(R2=0.999 9). The limit of detection was 0.01 μg/mL,the recovery range was 99.7% to 100.5%,and the relative standard deviation(RSD)range was 0.5% to 0.6%. The taurine content of brands A and B(dark eggs),brands C and D(native eggs),and ordinary eggs was 1.332,1.151,1.063,0.735,0.622 mg/100 g,respectively. The taurine content in the yolk of ordinary eggs was significantly lower than that in brands A and B(dark eggs)and brand C(native eggs,P<0.05),and slightly lower than that in brand D(native eggs,P>0.05). The method was simple,accurate,specific,and suitable for the determination of taurine content in eggs.

关 键 词：高效液相色谱法 牛磺酸含量 普通鸡蛋 乌鸡蛋 柴鸡蛋 

分 类 号：O657.72[理学—分析化学] TS253.7[理学—化学]