一株革兰氏阳性菌对猪去氧胆酸和猪胆酸生物转化的研究  

Study on the Biotransformation of Hyodesoxycholic Acid and Hyocholic Acid by a Gram-Positive Strain

作　　者：欧阳文凯 盛敏 蔡帅 李跃忠 李国军 邓家国 谢小国 万里平 李刚 OUYANG Wenkai;SHENG Min;CAI Shuai;LI Yuezhong;LI Guojun;DENG Jiaguo;XIE Xiaoguo;WAN Liping;LI Gang(Changde Yungang Biotechnology Co.,Ltd.,Hunan Changde 415001)

机构地区：[1]常德云港生物科技有限公司,湖南常德415001

出　　处：《生物化工》2021年第6期34-38,43,共6页Biological Chemical Engineering

摘　　要：猪胆在我国来源丰富,价格低廉,猪去氧胆酸(Hyodeoxycholic Acid,HDCA)和猪胆酸(Hyocholic Acid,HCA)作为猪胆的主要提取产物,提取工艺成熟,以这两种化合物为原料作为生物转化的研究具有很大的经济效益和社会效益。本研究以HDCA为唯一碳源的无机盐培养基筛选得到了125株菌,利用其中一株革兰氏阳性菌SX-1对HDCA和HCA进行了生物转化的研究。结果显示,菌株SX-1对HDCA没有明显转化作用;转化时间48 h时,对HCA有很强的转化作用,TLC和HPLC结果显示,HCA几乎被完全转化。经过波谱分析,转化产物为3,7-二酮基-6α-羟基-胆酸。Pig bile is rich in sources and low price,hyodesoxycholic acid(HDCA)and hyocholic acid(HCA)are the main extracts of pig bile,the research on HDCA and HCA as raw materials as biological transformation has great economic and social benefits.The inorganic salt culture screening with HDCA as the sole carbon source obtained 125 strains,study of biological transformation of HDCA and HCA using one of the gram-positive bacteria,SX-1.The results show,strain SX-1 has no significant transformation effect on HDCA;At the conversion time of 48 h,with a strong conversion effect on HCA.The TLC and HPLC results show,HCA is consumed almost completely.The identification results show the conversion product is 3,7-diketone-6α-hydroxyl-bile acid.

关 键 词：猪去氧胆酸 猪胆酸 生物转化 

分 类 号：TQ460.6[化学工程—制药化工]