枯草芽孢杆菌表面展示技术用于黏膜疫苗的研究进展  

Research progress on Bacillus subtilis surface display technology for mucosal vaccine

作　　者：李威杰 刘明刚[1,2] 李建臻 潘康成 LI Weijie;LIU Minggang;LI Jianzhen;PAN Kangcheng(College of Veterinary Medicine,Sichuan Agricultural University,Chengdu 611130,Sichuan,China;Fujian Luodong Bio-Technology Limited Compamy,Putian 351162,Fujian,China;Chengdu Agricultural College,Chengdu 611130,Sichuan,China)

机构地区：[1]四川农业大学动物医学院,四川成都611130 [2]福建洛东生物技术有限公司,福建莆田351162 [3]成都农业科技职业学院,四川成都611130

出　　处：《微生物学报》2022年第1期65-76,共12页Acta Microbiologica Sinica

基　　金：国家自然科学基金(31472130);四川省高等学校科技创新团队项目(动物源性食品安全创新团队)(KM406183.1);成都农业科技职业学院青年基金(20QN01)。

摘　　要：枯草杆菌全名枯草芽孢杆菌(Bacillus subtilis),因其优秀的益生特性及芽孢良好的抗逆性而备受研究者青睐,由于芽孢的特殊结构及独特的生理特性,是酶和免疫原等外源蛋白的理想锚定点。采用枯草杆菌进行芽孢表面展示被认为是表达高活性和高稳定性的外源蛋白的方法之一。本文主要对枯草杆菌芽孢表面展示抗原蛋白以生产黏膜疫苗的策略和应用前景进行综述。Bacillus subtilis was favored by researchers attributing to the excellent probiotic properties and strong stress resistance of spores.Owing to the special structure and unique physiological characteristics,the spores are an ideal place for foreign proteins such as enzymes and immunogens to anchor.B.subtilis spore surface display is one of the methods to express foreign proteins with high activity and stability.This article mainly reviewed the strategies and application prospects of displaying antigen proteins on the surface of B.subtilis spores to produce mucosal vaccines.

关 键 词：枯草芽孢杆菌 芽孢表面展示 抗原蛋白 黏膜免疫 

分 类 号：S859.797[农业科学—临床兽医学]