Tangeretin inhibits fungal ferroptosis to suppress rice blast  

作　　者：Meiling Liang Huijuan Ye Qing Shen Xianya Jiang Guobing Cui Wenxiang Gu Lian-Hui Zhang Naweed I.Naqvi Yi Zhen Deng 

机构地区：[1]State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources,Guangdong Province Key Laboratory of Microbial Signals and Disease Control,Integrative Microbiology Research Center,South China Agricultural University,Guangzhou 510642,China [2]Zhaoqing Food Inspection Institute,Zhaoqing 526000,China [3]Temasek Life Sciences Laboratory,Singapore 117604,Singapore [4]Yangjiang Institute of Agricultural Sciences,Yangjiang 529500,China [5]Department of Applied Chemistry,College of Materials and Energy,South China Agricultural University,Guangzhou 510642,China [6]Department of Biological Sciences,National University of Singapore,Singapore 117558,Singapore

出　　处：《Journal of Integrative Plant Biology》2021年第12期2136-2149,共14页植物学报（英文版）

基　　金：supported by the National Natural Science Foundation of China(32022070);Key Realm R&D Program of Guangdong Province(2020B0202090001);China Postdoctoral Science Foundation(2020M682723);by Temasek Life Sciences Laboratory(Singapore)。

摘　　要：Flavonoids are polyphenolic secondary metabolites that function as signaling molecules,allopathic compounds,phytoalexins,detoxifying agents and antimicrobial defensive compounds in plants.Blast caused by the fungus Magnaporthe oryzae is a serious disease affecting rice cultivation.In this study,we revealed that a natural flavonoid,tangeretin,substantially delays the formation of M.oryzae appressoria and blocks the development of blast lesions on rice plants.Our data suggest that tangeretin has antioxidant activity that interferes with conidial cell death/ferroptosis,which is critical for M.oryzae pathogenicity.Tangeretin showed a ferroptosis inhibition efficacy comparable to the well-established liproxstatin-1.Furthermore,overexpression of the NADPH oxidases NOX1 or NOX2 significantly decreased sensitivity toward tangeretin treatment,suggesting Nox-mediated lipid peroxidation as a possible target for tangeretin in regulating redox signaling and ferroptosis in M.oryzae.Our nursery and field tests showed that application of tangeretin can effectively mitigate overall disease symptoms and prevent leaf blast.Our study reveals the plant-derived fungal ferroptosis inhibitor tangeretin as a potential and novel antifungal agrochemical for the sustainable prevention of the devastating blast disease in important cereal crops.

关 键 词：antifungal agrochemical antioxidant APPRESSORIUM autophagy cell death ferroptosis lipid peroxidation NOX rice blast tangeretin 

分 类 号：S435.111.41[农业科学—农业昆虫与害虫防治]