TaNPR1在小麦抵抗叶锈菌过程中的作用  

Role of TaNPR1 in wheat resistance to Puccinia triticina

作　　者：李姗 乔金柱 李烨 贾丁柔 刘刚[1] 王冬梅[1] LI Shan;QIAO Jinzhu;LI Ye;JIA Dingrou;LIU Gang;WANG Dongmei(State Key Laboratory of North China Crop Improvement and Regulation/Key Laboratory of Hebei Province for Plant Physiology and Molecular Pathology/College of Life Sciences,Hebei Agricultureal University,Baoding,071001,China)

机构地区：[1]河北农业大学华北作物改良与调控国家重点实验室/河北省植物生理与分子病理学重点实验室/生命科学学院,河北保定071001

出　　处：《河北农业大学学报》2022年第1期1-6,共6页Journal of Hebei Agricultural University

基　　金：国家自然科学基金项目(31171472、31871548);河北省自然科学基金资助项目(C2019204280);2020年度河北省引进留学人员资助项目(C20200331).

摘　　要：叶锈病是一种影响小麦(Triticum aestivum)产量的重要病害,挖掘广谱有效的抗病基因有利于促进小麦品种改良。NPR(Nonexpressor of pathogenesis-related genes,NPR)是一类具有广谱抗病作用的关键因子,本研究分析了TaNPR1在抵抗叶锈菌过程中的功能。通过qRT-PCR(quantitative Real-time-Polymerase Chain Reaction)检测,亚细胞定位观察,VIGS技术沉默TaNPR1后试验,表明TaNPR1影响寄主HR(Hypersensitive reaction)的产生,在小麦抵抗叶锈菌侵染过程中发挥正调控作用。Wheat leaf rust is an important disease that affects the yield.Identification of broad-spectrum disease-resistant genes is beneficial to the improvement of wheat varieties.NPR is a key factor in broad-spectrum disease resistance.In this article,we analyzed the function of TaNPR1 in the process of wheat resistance to Puccinia triticina.Expression analysis showed that TaNPR1 expression was induced by Puccinia triticina inoculation at the initial stage and the response was stronger in the affinity combination(Tc×260)compared with the incompatible combination(TcLr26×260).Subcellular localization showed that TaNPR1 was localized in the cytoplasm and nucleus.,The VIGS technique was applied on plants of the incompatible combination to silence the TaNPR1 gene.The silencing plants displayed significantly larger area of HR at the infection point with significantly increased numbers of HMC.These findings suggested that TaNPR1 affects the generation of host HR and plays a positive role in the process of wheat resistance to leaf rust infection.

关 键 词：小麦 叶锈菌 TaNPR1 过敏性反应 

分 类 号：S512.1[农业科学—作物学]