体外受精-胚胎移植技术中囊胚培养密度对胚胎发育潜能的影响  

Effect of blastocyst culture density on embryo development potential of in vitro fertilization-embryo transfer technology

作　　者：陈棪焜[1] 冼卓杰[1] 池霖生[1] 黄建洲[1] 马文敏[1] CHEN Yankun;XIAN Zhuojie;CHI Linsheng;HUANG Jianzhou;MA Wenmin(Assisted Reproductive Technology Center,Foshan Maternal and Child Health Hospital,Guangdong Province,Foshan528000,China)

机构地区：[1]广东省佛山市妇幼保健院辅助生殖技术中心,广东佛山528000

出　　处：《中国当代医药》2022年第2期109-112,共4页China Modern Medicine

基　　金：广东省佛山市卫生和计生局医学科研课题项目(20190123)。

摘　　要：目的分析体外受精-胚胎移植技术(IVF-ET)中囊胚培养密度对胚胎发育潜能的影响。方法选取2019年1月至12月佛山市妇幼保健院接受体外受精移植的235例体外受精胚胎移植患者为研究对象,将顺利移植的1181个裂卵期胚胎按照囊胚培养密度差异分为四组,单独培养为A组,2~3个集中培养为B组,4~5个集中培养为C组,6个以上集中培养为D组,其中A组培养囊胚共269个,B组培养囊胚共298个,C组培养的囊胚共304个,D组培养囊胚共310个。比较四组的囊胚形成率、可利用囊胚率、优质囊胚率、囊胚等级差异、囊胚着床率及临床妊娠率。结果C组的囊胚形成率、可利用囊胚率、优质囊胚率均高于A、B、D组,差异有统计学意义(P<0.05);C组的囊胚4~6期占比高于A、B、D组,差异有统计学意义(P<0.008)。四组胚胎共形成778个胚囊,C组形成囊胚的囊胚着床率、临床妊娠率高于A、B、D组,差异有统计学意义(P<0.008)。结论囊胚培养密度为4~5个囊胚集中培养时囊胚的发育潜能好,各方面结果均较其余数量培养结果更好,囊胚培养时密度不宜过大也不宜过小。Objective To study the effect of blastocyst culture density on embryo development potential of in vitro fertilization embryo transfer(IVF-ET).Methods A total of 235 patients with IVF-ET Foshan Maternal and Child Health Hospital from January 2019 to December 2019 were selected as subjects,and the 1181 successfully transplanted cleavage embryos were divided into four groups according to the difference in blastocyst culture density,individual cultures were grown in group A,2 to 3 in group B,4 to 5 in group C,and more than 6 in group D,there were 269 blastocysts in group A,298 blastocysts in group B,304 blastocysts in group C,and 310 blastocysts in group D.Blastocym formation rates,available blastocyst rates,high quality blastocyst level differences,blastocyst implantation rates and clinical pregnancy rates in the 4 groups were compared.Results The blastocyst formation rate,usable blastocyst rate,and high-quality blastocyst rate in group C were higher than those in groups A,B,and D,and the differences were statistically significant(P<0.05).Blastocysts accounted for stages 4 to 6 in group C was higher than that of groups A,B,and D,the differences were statistically significant(P<0.008).Four groups of embryos form a total of 778 embryo sacs,the blastocyst implantation rate and clinical pregnancy rate of blastocyst formation in group C were higher than those of groups A,B,and D,the differences were statistically significant(P<0.008).Conclusion The blastocyst culture density is 4 to 5 blastocysts.The development potential of blastocysts is good.The results in all aspects are better that those of other quantities.Better,it means that the density of blastocyst culture should not be too high or too small.

关 键 词：体外受精 胚胎移植技术 囊胚密度 胚胎发育潜能 

分 类 号：R321[医药卫生—人体解剖和组织胚胎学]