GmELF3s调控大豆开花时间和生物钟节律的功能分析  

Function analysis of GmELF3s in regulating soybean flowering time and circadian rhythm

作　　者：徐昕 秦超 赵涛[1] 刘斌[1] 李宏宇[1] 刘军[1] XU Xin;QIN Chao;ZHAO Tao;LIU Bin;LI Hong-Yu;LIU Jun(Institute of Crop Sciences,Chinese Academy of Agricultural Sciences,Beijing 100081,China)

机构地区：[1]中国农业科学院作物科学研究所,北京100081

出　　处：《作物学报》2022年第4期812-824,共13页Acta Agronomica Sinica

基　　金：中国农业科学院科技创新工程-作物生物信息学及应用项目(2060302-2-20);大豆藏粮于技项目(CAAS-ZDRW202003)资助。

摘　　要：大豆是典型的短日照作物,光周期的敏感性严重影响大豆的开花时间和产量,制约大豆的种植范围,但调控大豆光周期和生物钟节律的机制尚不十分清楚。在模式植物拟南芥中,ELF3与ELF4、LUX一起,形成ELF4-ELF3-LUX(Evening Complex,EC)生物钟晚间复合物,在生物钟节律和开花时间调控等方面发挥重要作用。本研究通过CRISPR/Cas9基因编辑系统获得大豆Gmelf3a/j、Gmelf3b-1和Gmelf3b-2的突变体材料。通过观察Gmelf3a/j、Gmelf3b-1和Gmelf3b-2各突变体材料在短日照和长日照下的开花时间发现,GmELF3b-1在长日照下对大豆开花时间起调控作用;通过观察非纯合双突变体的表型发现,GmELF3a/J与GmELF3b-1和GmELF3b-2之间在调控大豆开花时间方面存在功能冗余。通过qRT-PCR对大豆生物钟节律相关基因的表达进行检测发现,GmCAB、GmPRR9a和GmPRR7a的表达模式发生改变,这表明GmELF3a/J、GmELF3b-1和GmELF3b-2可能是通过GmPRR9a和GmPRR7a对大豆生物钟节律和开花时间进行调控。Soybean is a typical short-day crop.Photoperiod sensitivity seriously affects flowering time,yield,and planting range of soybean,but the mechanism underlying photoperiod and circadian rhythm regulation is still unclear.In Arabidopsis thaliana,ELF3,together with ELF4 and LUX,forms the ELF4-ELF3-LUX complex(Evening Complex,EC),which plays an important role in circadian rhythm and flowering time regulation.In this study,soybean mutants of Gmelf3a/j,Gmelf3b-1,and Gmelf3b-2 were obtained by CRISPR/Cas9 gene editing system.We found that GmELF3b-1 regulated the flowering time in soybean under long-day conditions by observing flowering phenotypes in these mutants.The phenotypes of heterozygous double mutants revealed that there was functional redundancy among GmELF3a/J,GmELF3b-1,and GmELF3b-2 in regulating flowering time of soybean.Through detecting the expression of circadian related genes in soybean by using qRT-PCR,it was found that the relative expression patterns of GmCAB,GmPRR9a,and GmPRR7a were changed in soybean.In summary,these results suggested that GmELF3a/J,GmELF3b-1,and GmELF3b-2 may regulate the circadian rhythm and flowering time through GmPRR9a and GmPRR7a in soybean.

关 键 词：光周期 生物钟节律 开花时间 晚间复合物 ELF3 

分 类 号：S565.1[农业科学—作物学]