过表达ZmCIPKHT基因增强植物耐热性  

Overexpression of ZmCIPKHT enhances heat tolerance in plant

作　　者：许静 高景阳 李程成 宋云霞 董朝沛 王昭 李云梦 栾一凡 陈甲法 周子键[2] 吴建宇[1,2] XU Jing;GAO Jing-Yang;LI Cheng-Cheng;SONG Yun-Xia;DONG Chao-Pei;WANG Zhao;LI Yun-Meng;LUAN Yi-Fan;CHEN Jia-Fa;ZHOU Zi-Jian;WU Jian-Yu(College of Agriculture,Henan Agricultural University,Zhengzhou 450002,Henan,China;College of Life Science,Henan Agricultural University,Zhengzhou 450002,Henan,China)

机构地区：[1]河南农业大学农学院,河南郑州450002 [2]河南农业大学生命科学学院,河南郑州450002

出　　处：《作物学报》2022年第4期851-859,共9页Acta Agronomica Sinica

基　　金：河南省自然科学基金重点项目(162300410130)资助。

摘　　要：高温胁迫对植物正常生长发育及产量的影响越来越显著。为了适应外界环境的变化,植物进化出了一系列应对高温胁迫的分子遗传机制。类钙调磷酸酶B蛋白(CBL)互作蛋白激酶(CIPK),在ABA信号转导途径上积极参与植物对高温胁迫的响应。在前期全基因组关联分析的基础上,本实验克隆了一个与玉米耐高温性状相关的候选基因ZmCIPKHT,qRT-PCR结果表明ZmCIPKHT基因受高温胁迫的显著诱导。室内表型鉴定的实验发现过表达ZmCIPKHT的转基因拟南芥植株在高温胁迫下,比野生型的存活率显著提高,生长状态更好。玉米原生质体瞬时转化实验证明ZmCIPKHT蛋白定位于细胞核中。酵母双杂交实验验证了ZmCIPKHT蛋白与玉米CBLs家族中的ZmCBL4蛋白存在互作关系。同时,ZmCIPKHT转基因拟南芥在高温胁迫条件下,脱落酸(ABA)通路相关基因的表达水平有其相应的变化,揭示了ZmCIPKHT可能依赖于ABA信号转导通路来增强植物的耐热性。这些结果为解析玉米CBL-CIPK信号通路依赖于ABA途径对植物非生物胁迫响应的分子机制提供了新的实验根据。The effects of high temperature stress on the normal growth and yield of plants is more and more serious.To adapt the changes of external environment,plants have evolved a series of molecular genetic mechanisms to respond to high temperature stress.The calcineurin B-like protein(CBL)interacting protein kinase(CIPK)is actively involved in response to high temperature stress depended on ABA signal transduction pathway in plants.Based on previous genome-wide association analysis,a candidate gene ZmCIPKHT related to maize high temperature tolerance was cloned in this study.Real-time quantitative PCR results showed that ZmCIPKHT gene was significantly induced by high temperature stress.Transient transformation of maize protoplasts revealed that ZmCIPKHT was localized in the nucleus.Overexpressing ZmCIPKHT plants of transgenic Arabidopsis thaliana had significantly higher survival rate and better growth status than wild type under high temperature stress.The yeast two-hybrid experiment confirmed that the interaction between ZmCIPKHT protein and ZmCBL4 protein in maize CBLs family.The relative expression levels of genes related to abscisic acid(ABA)pathway in transgenic Arabidopsis thaliana with ZmCIPKHT under high temperature stress were changed accordingly,indicating that the regulations of ZmCIPKHT genes under high temperature stress were in the ABA-dependent pathway.These results provide a new experimental basis for elucidating the molecular mechanism of maize CBL-CIPK signaling pathway dependent on ABA pathway to abiotic stress in plants.

关 键 词：玉米 耐热性 蛋白激酶 CBL-CIPK ABA信号通路 

分 类 号：S513[农业科学—作物学]