五味子乙素对铝中毒大鼠学习记忆及突触相关蛋白表达影响的研究  

Effects of Schisandrin B on the learning and memory and the expression of synapse-related proteins in rats with aluminum intoxication

作　　者：程厚之 张丽凤 梁祚仁[1] 廖素婵 李艳丽 莫颂轶[1] 黄彦峰[1] 余双全 黄俊杰[1] Cheng Houzhi;Zhang Lifeng;Liang Zuoren;Liao Suchan;Li Yanli;Mo Songyi;Huang Yanfeng;Yu Shuangquan;Huang Junjie(School of Basic Medicine,Youjiang Medical University for Nationalities,Baise 533000,Guangxi,China)

机构地区：[1]右江民族医学院基础医学院,广西百色533000

出　　处：《右江民族医学院学报》2021年第6期714-718,共5页Journal of Youjiang Medical University for Nationalities

基　　金：广西自然科学基金项目(2018GXNSFAA281142);右江民族医学院高层次人才科研课题(yy2020gcky036)。

摘　　要：目的 研究五味子乙素(Schisandrin B,Sch B)对慢性铝中毒大鼠海马和大脑皮层中突触相关蛋白表达及学习记忆功能的影响,并探讨其分子机制。方法 用AlCl_(3)连续喂养大鼠3个月建立慢性铝中毒动物模型,动物随机分为5个组,即模型组、阳性对照组、五味子乙素低剂量组、中剂量组、高剂量组,阳性对照组用吡拉西坦按40mg/kg剂量灌胃给药,五味子乙素低剂量组、中剂量组、高剂量组,分别用五味子乙素按10mg/kg、20mg/kg、40mg/kg剂量进行灌胃,各组每天灌胃1次,连续灌胃30d;另设空白对照组,每组各10只。用Morris水迷宫定位航行实验记录大鼠找到平台的游泳路径轨迹,免疫组化和Western Blot分别检测突触素(SYN)、突触后致密蛋白95(PSD-95)在海马组织和大脑皮层中的表达水平。结果 模型组大鼠运动轨迹趋向性较差,偏离平台;与模型组比较,五味子乙素各剂量组大鼠运动轨迹更多趋向于目标平台。免疫组化结果显示,铝中毒模型组海马和大脑皮层中SYN、PSD-95表达水平均较空白组明显降低(P <0.01);而高剂量组SYN、PSD-95在海马和大脑皮层中表达水平比模型组的明显增多(P <0.01)。Western Blot结果显示,铝中毒模型组海马组织和大脑皮层中SYN、PSD-95蛋白相对表达量均比空白对照组的明显降低(P <0.01);Sch B高剂量组PSD-95、SYN蛋白相对表达量比模型组均明显升高(P <0.05)。结论 Sch B能改善慢性铝中毒大鼠学习记忆能力,可能与通过上调海马组织和大脑皮层中SYN和PSD-95蛋白表达有关。Objective To investigate the effects of Schisandrin B(Sch B)on synapse-related protein expression in hippocampus and cerebral cortex as well as the learning and memory function of rats with chronic aluminum intoxication,and to discuss the molecular mechanism.Methods Rats were fed with AlCl_(3) continuously for 3 months to establish the animal model of chronic aluminum intoxication.The rats were randomly divided into 5 groups:the model group,the positive control group,the low-,medium-and high-dose Sch B groups.Rats in the positive control group were given piracetam at 40 mg/kg by intragastric administration.Rats in the low-dose,medium-dose and high-dose groups were given Sch B by intragastric administration at 10 mg/kg,20 mg/kg and 40 mg/kg,respectively.Each group was given medicine intragastrically once a day for 30 days.The blank control group was also established and there were 10 rats in each group.The navigation experiment of Morris water maze was carried out to record the swimming path trajectory of the rats finding the platform.The expression of synaptophysin(SYN)and postsynaptic dense protein 95(PSD-95)in hippocampus and cerebral cortex were detected by immunohistochemistry and Western Blot,respectively.Results The movement trajectory of rats in the model group tended to be poor and deviated from the platform.Compared with that of the model group,the trajectory of rats in each Sch B group was toward the target platform more closely.Immunohistochemistry results showed that the expressions of SYN and PSD-95 in hippocampus and cerebral cortex of the aluminum intoxication model group were significantly lower than those of the control group(P<0.01),and the expressions of SYN and PSD-95 in hippocampus and cerebral cortex of the high-dose group were significantly higher than those of the model group(P<0.01).The results of Western Blot showed that the relative expressions of SYN and PSD-95 protein in the hippocampus and cerebral cortex of the aluminum intoxication model group were significantly lower than those of

关 键 词：五味子乙素 慢性铝中毒 学习记忆能力 突触相关蛋白 大脑皮层 海马 

分 类 号：R285.5[医药卫生—中药学]