痛风康对急性痛风性关节炎大鼠的治疗作用及机制研究  

Therapeutic effect and mechanism of Tongfengkang on rats with acute gouty arthritis

作　　者：尤元梅 袁松[2] 徐凌 You Yuanmei;Yuan Song;Xu Ling(North Anhui Health Vocational College,Suzhou 234000,Anhui,China;Suzhou Hospital of Traditional Chinese Medicine,Suzhou 234000,Anhui,China)

机构地区：[1]皖北卫生职业学院,安徽宿州234000 [2]安徽省宿州市中医院,安徽宿州234000

出　　处：《右江民族医学院学报》2021年第6期736-740,共5页Journal of Youjiang Medical University for Nationalities

基　　金：皖北卫生职业学院校级科学研究重点项目(WZK201903)。

摘　　要：目的 通过应用中药组方痛风康对急性痛风性关节炎大鼠进行治疗,探讨痛风康治疗急性痛风性关节炎的可能机制。方法 将60只雄性健康SD大鼠随机分为6组,分别为正常组、模型组、吲哚美辛组、痛风康高剂量组、痛风康中剂量组、痛风康低剂量组,每组10只。痛风康高、中、低剂量组采用痛风康配置的不同浓度中药灌胃,吲哚美辛组采用吲哚美辛灌胃,正常组、模型组采用生理盐水灌胃,连续给药8d。灌胃5d后,除正常组外,其余各组采用尿酸钠溶液注射大鼠右踝关节腔造模,诱导急性痛风性关节炎模型。造模前1天检测大鼠体质量,造模后不同时间检测大鼠体质量、踝关节肿胀度、步态评分。灌胃8d后,ELISA法检测大鼠血清中细胞间黏附因子-1(ICAM-1)、肿瘤坏死因子(TNF-α)、白细胞介素-1β(IL-1β)的含量。结果 ①与正常组相比,造模后其余各组大鼠步态改变,踝关节肿胀明显,呈急性炎性红肿,活动障碍明显;②与模型组相比,治疗后痛风康高剂量组、痛风康中剂量组及痛风康低剂量组大鼠踝关节肿胀度减轻(P <0.05);大鼠步态评分下降(P <0.05);血清中ICAM-1、TNF-α、IL-1β含量降低(P <0.05)。结论 中药组方痛风康治疗大鼠急性痛风性关节炎具有改善大鼠关节症状,降低实验室检测指标的作用,其机制可能与下调TLR4/NF-κB通路下游的炎症因子有关。Objective To investigate the possible mechanism of traditional Chinese medicine(TCM)formula Tongfengkang in the treatment of acute gouty arthritis by treating rats with acute gouty arthritis.Methods Sixty healthy male SD rats were randomly divided into 6 groups:the normal group,the model group,the indomethacin group,high-dose Tongfengkang group,medium-dose Tongfengkang group and low-dose Tongfengkang group,with 10 rats in each group.Rats in high-,medium-and low-dose Tongfengkang groups were given intragastrically different concentrations of Chinese medicine made of Tongfengkang.Rats in the indomethacin group were given indomethacin by intragastric administration.The normal group and the model group were given normal saline by intragastric administration.All the rats were given medicine following the above ways for 8 consecutive days.After 5 days of intragastric administration,the solution of sodium urate was injected into the right ankle cavity of rats to induce the model of acute gouty arthritis,except for the normal group.The body mass of rats was measured one day before modeling,and the body mass,ankle swelling and gait scores were determined at different times after modeling.After 8 days of intragastric administration,Elisa was adopted to detect the levels of intercellular adhesion molecule-1(ICAM-1),tumor necrosis factor(TNF-α)and interleukin-1β(IL-1β)in serum of rats.Results①Compared with those of the normal group,rats in other groups showed significant gait changes,ankle swelling,acute inflammatory redness and activity disorders after modeling.②Compared with that of the model group,the swelling of ankle joint was relieved in rats of the high-dose Tongfengkang group as well as the medium-dose Tongfengkang group and the low-dose Tongfengkang group after treatment(P<0.05).And there was an decrease in the gait scores of these groups(P<0.05)as well as inserum ICAM-1,TNF-αand IL-1βof the rats(P<0.05).Conclusion Tongfengkang,a kind of TCM formula,can improve the joint symptoms and reduce the laboratory t

关 键 词：痛风康 吲哚美辛 痛风性关节炎 

分 类 号：R285[医药卫生—中药学]