利胆合剂君臣组分的药效物质基础研究  

Study on medicinal effective material base of monarch and minister components in Li-Dan-He-Ji

作　　者：丘燕燕 鄢素琪[2] 周俪姗[2] 熊小丽[2] 江治霞[2] 易巍 秦欢 Qiu Yanyan;Yan Suqi;Zhou Lishan;Xiong Xiaoli;Jiang Zhixia;Yi Wei;Qin Huan(Clinical College of Traditional Chinese Medicine,Hubei University of Chinese Medicine,Wuhan 430065,Hubei,China;Department of Integrated Chinese and Western Medicine,Wuhan Children's Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430016,Hubei,China;Department of Laboratory,Wuhan Children's Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430016,Hubei,China)

机构地区：[1]湖北中医药大学中医临床学院,湖北武汉430065 [2]华中科技大学同济医学院附属武汉儿童医院中西医结合科,湖北武汉430016 [3]华中科技大学同济医学院附属武汉儿童医院检验科,湖北武汉430016

出　　处：《中国中西医结合急救杂志》2021年第5期601-605,共5页Chinese Journal of Integrated Traditional and Western Medicine in Intensive and Critical Care

基　　金：国家自然科学基金面上项目(81574024);湖北省卫生和计划生育委员会中医药中西医结合科研项目(2017HZ-Y35)。

摘　　要：目的建立利胆合剂君臣组分指纹图谱,鉴定其主要活性物质,研究利胆合剂君臣组分的药效物质基础。方法制备利胆合剂君臣组分基准溶液,采用超高效液相色谱法(UPLC)测定利胆合剂君臣组分的指纹图谱,并开展方法学研究。使用Waters Acquity UPLC HSS T3色谱柱(粒径1.8μm,100 mm×2.1 mm),以乙腈、1%磷酸-水溶液为流动相进行梯度洗脱,柱温35℃,进样量1μL,流速0.3 mL/min,检测波长210~600 nm全扫描。结果建立的利胆合剂君臣组分UPLC指纹图谱分析显示,各供试液样品指纹图谱峰型一致,可判断茵陈、连翘、熟大黄的药材饮片、提取物和颗粒剂3种样品的药材质量相当;鉴定了绿原酸、连翘苷、连翘脂苷A、芦荟大黄素、大黄酸、大黄素、大黄酚和大黄素甲醚8种主要活性物质。10个批次的利胆合剂君臣组分颗粒剂成分稳定,绿原酸和连翘苷在相应浓度范围内与峰面积的线性关系良好,线性回归方程分别为y=3954.10x+4362.00(R^(2)=0.9995)和y=2097.70x+349.75(R^(2)=0.9999);加样回收率为99.30%~102.11%,相对标准偏差(RSD)为0.05%~0.23%。结论利胆合剂君臣组分的UPLC指纹图谱和主要活性物质鉴定表征了利胆合剂整体与部分的差异,为利胆合剂组方的成药制剂开发及药物作用机制研究提供实验依据,也为中药复方制剂的质量控制提供方法学借鉴。Objective To establish the fingerprints of monarch and minister components in Li-Dan-He-Ji(LDHJ)in order to identify their main active substances and study medicinal effective material basis of LDHJ monarch and minister components.Methods The standardized solutions of monarch and minister components in LDHJ were prepared.The fingerprints of monarch and minister components in LDHJ were determined by ultra performance liquid chromatography(UPLC)and the methodology was studied.Waters Acquity UPLC HSS T3(particle size of 1.8μm,100 mm×2.1 mm)chromatographic column was used to separate the components,and the mobile phase of acetonitrile-1%phosphoric acid liquid solution was applied to carry out gradient elution;the flow rate 0.3 mL/min,the column temperature of 35℃,the sample size of 1μL and the detection wavelength ranging from 210-600 nm were set up for full scanning.Results The UPLC fingerprints of monarch and minister components in LDHJ were established,the fingerprint peak types of the sample solutions provided for testing were consistent,which could judge that the medicinal material qualities of the three samples of Yinchen,Forsythia suspensa and cooked rhubarb prepared as medicinal piece,extract or granule types were equivalent;8 main active substances,including chlorogenic acid,forsythoside-A,forsythin,aloe-emodin,rhein,emodin,chrysophanol,and physcion were identified.The compositions of monarch and minister components in LDHJ in 10 different granule batches were stable,under corresponding concentration range,the linear relationships of chlorogenic acid and forsythin with peak areas were good,and the linear regression equations were y=3954.10x+4362.00(R^(2)=0.9995)and y=2097.70x+349.75(R^(2)=0.9999),respectively.The average recovery rate range of added sample was 99.30%-102.11%and relative standard deviation(RSD)range was 0.05%-0.23%.Conclusion The UPLC fingerprints of LDHJ monarch and minister components and the identification of the main active substances in LDHJ characterize the overall and partial diff

关 键 词：利胆合剂 超高效液相色谱 指纹图谱 活性物质 

分 类 号：R28[医药卫生—中药学]