有核红细胞全血分选微管芯片实验研究  

Experimental Study on Separation of Fetal Nucleated Red Blood Cells from the Whole Blood Using Microfluidic Chip

作　　者：刘洁 张佳荣 庄研[3] 贺银燕[3] 张晟泓 陈鑫[1] 龚晓波[1] LIU Jie;ZHANG Jiarong;ZHUANG Yan;HE Yinyan;ZHANG Shenghong;CHEN Xin;GONG Xiaobo(Department of Engineering Mechanics,Shanghai Jiao Tong University,Shanghai 200240,China;Department of Gynaecology and Obstetrics,Zhongshan Hospital,Fudan University,Shanghai 200032,China;Department of Gynaecology and Obstetrics,Shanghai General Hospital,Shanghai 200080,China)

机构地区：[1]上海交通大学工程力学系,上海200240 [2]复旦大学附属中山医院妇产科,上海200032 [3]上海市第一人民医院妇产科,上海200080

出　　处：《医用生物力学》2021年第6期903-909,共7页Journal of Medical Biomechanics

基　　金：国家自然科学基金面上项目(11872040,12072198);国家重点研发计划(2017YFE0117100)。

摘　　要：目的设计微流控芯片分离全血中胎儿有核红细胞(fetal nucleated red blood cell,fNRBCs),实现有核红细胞的快速便捷获取。方法利用有核细胞在血流作用下的边集效应以及细胞抗原抗体特异性黏附特点,设计微管流控芯片,分离全血中的fNRBCs。以脐血全血为例,通过免疫荧光计数,分析不同剪切率对fNRBCs富集效果的影响。结果相较于简单静置黏附,增加剪切率可以增加直微管黏附有核红细胞的数量,细胞的富集效果随血流剪切率的增大先增大后减小。结论利用直微管能够实现fNRBCs的全血快速有效捕获。研究结果为无创产前诊断的发展以及胎儿细胞转移机制的探究提供实验参考。Objective To seperate fetal nucleated red blood cells(fNRBCs)from the whole maternal peripheral blood effectively by designing a circular channel microfluidic chip.Methods A microfluidic chip is designed by utilizing the margination in blood flow and the specific adhesion characteristics of immuno-agent anti-CD147.With the whole umbilical cord blood,the effects of different shear forces on the enrichment of fNRBCs was studied by immunofluorescence counting.Results Increasing shear rate in microfluidic chip could improve the number of captured fNRBCs compared with the static adhesion.With the increase of shear rate of blood flow,the number of the captured cells increased at first,and then decreased.Conclusions The use of microfluid chip can effectively seperate fNRBCs from the whole blood.The results provide an experimental reference for the non-invasive prenatal diagnosis research and the exploration on the mechanism of fetal cell migration.

关 键 词：非侵入性产前诊断 胎儿有核红细胞 滚动黏附 微管流控芯片 

分 类 号：R318.01[医药卫生—生物医学工程]