基于原子力显微镜研究固-液界面单链DNA结合蛋白纤维分形结构的形成机制  

Formation mechanism of single-stranded DNA binding protein fibers fractal structure at liquid-solid interface studied by atomic force microscopy

作　　者：邢春燕 张苗苗 乔海燕 唐纪琳[1] 张柏林[1] XING Chunyan;ZHANG Miaomiao;QIAO Haiyan;TANG Jilin;ZHANG Bailin(State Key Laboratory of Electroanalytical Chemistry,Changchun Institute of Applied Chemistry,Chinese Academy of Sciences,Changchun 130022,China;Shaanxi Anke Safety Production Technology Research Institute Company Limited,Xi’an 710065,China;College of Petrochemical Engineering,Liaoning Shihua University,Fushun 113001,China)

机构地区：[1]中国科学院长春应用化学研究所电分析化学国家重点实验室,长春130022 [2]陕西安科安全生产技术研究所有限公司,西安710065 [3]辽宁石油化工大学石油化工学院,抚顺113001

出　　处：《黑龙江大学自然科学学报》2021年第6期664-671,共8页Journal of Natural Science of Heilongjiang University

基　　金：Supported by the National Natural Science Foundation of China(20975096)。

摘　　要：单链DNA结合蛋白(SSBP)可以在修饰了1-十六烷基硫醇单层膜的金基底(HDT/Au)上由4-氨基噻吩(4-ATP)诱导自组装形成原纤维。利用原子力显微镜对SSBP自组装形成的分形结构进行了纳米尺度表征和成像,以及结构分析。结果显示,在HDT/Au基底上,酰胺类物质可以诱导蛋白质纤维的形成,而烷基硫醇或羧基硫醇不能在HDT/Au底物上诱导蛋白质原纤维的形成。这表明正电荷酰胺与蛋白质之间的静电力为蛋白质原纤维的自组装提供了促进聚合的驱动力。酰胺类物质诱导分枝状蛋白质纤维的形成,而不是更致密的单丝紧密堆积,为发展一种全新的、空间可控的蛋白质界面自组装方法提供了依据。Single-stranded DNA binding protein(SSBP)could self-assemble into fibrils by 4-aminothiophene(4-ATP)on a gold substrate modified with 1-hexadecyl mercaptan monolayer membrane(HDT/Au).The nanoscale characterization and imaging of the branched patterns formed by the self-assembly of proteins were observed via atomic force microscopy(AFM)for structural analysis.The results showed that amides could induce the formation of protein fibers on HDT/Au substrates,while alkanethiols or carboxythiols could not.It suggested that electrostatic forces between positively charged amides and proteins provided the driving forces to promote aggregation for the self-assembly of protein fibrils.The formation of branched-like protein fibrils rather than the more compact close-packing of single filaments induced by amide substances might provide a basis for the development of a new and spatially controllable protein interface self-assembly method.

关 键 词：自组装 原子力显微镜 4-氨基噻吩 单链DNA结合蛋白 纤维 

分 类 号：O651.2[理学—分析化学]