耐亚胺培南流感嗜血杆菌的临床特点与耐药性分析  

Analysis of the clinical characteristics and drug resistance of imipenem-resistant Haemophilus influenzae

作　　者：邢凡凡 黄锦月 王丽 李惠娟 叶海燕 孙林琳 邓超文 劳锦辉 陈福和 XING Fanfan;HUANG Jinyue;WANG Li;LI Huijuan;YE Haiyan;SUN Linlin;DENG Chaowen;LAO Jinhui;CHEN Fuhe(Department of Clinical Microbiology and Infection Control,the University of Hong Kong-Shenzhen Hospital,Shenzhen,Guangdong 518053,China)

机构地区：[1]香港大学深圳医院临床微生物及感染控制科,广东深圳518053

出　　处：《检验医学与临床》2022年第2期153-156,共4页Laboratory Medicine and Clinic

基　　金：广东省深圳市医疗卫生三名工程项目(SZSM201911014)。

摘　　要：目的探究耐亚胺培南流感嗜血杆菌(Hi_Ir)的临床特点与耐药性。方法对该院微生物实验室分离的30株Hi_Ir分别进行纸片扩散法(K-B法)与Epsilometer测试法(E-test法)药敏试验、β-内酰胺酶检测及血清型鉴定,使用医院信息系统检索相关临床资料,分析菌株的临床特征。结果30株Hi_Ir菌株分离自28例患者,其中男21例,女7例;年龄3个月至85岁;0~<15岁患者所占比例最高,为57.14%。30株Hi_Ir均分离自呼吸道标本,其中有9株为致病菌,14株为呼吸道定植菌,其余7株为作用不明菌。有12份标本同时检出其他呼吸道病原微生物。β-内酰胺酶阳性与阴性菌株各15株。两种药敏试验方法检测结果显示,30株Hi_Ir对头孢曲松、左氧氟沙星均敏感,K-B法检测出氨苄西林敏感株7株,K-B法检测出Hi_Ir菌株对美罗培南均敏感,但E-test法检测出4株Hi_Ir对美罗培南耐药。K-B法与E-test法检测的氨苄西林、阿莫西林/克拉维酸、美罗培南的耐药率比较,差异均无统计学意义(P>0.05)。结论该院分离的Hi_Ir主要引起呼吸道感染,对头孢曲松、左氧氟沙星、美罗培南仍有高敏感性。Objective To explore the clinical characteristics and drug resistance of imipenem-resistant Haemophilus influenzae(Hi_Ir).Methods The 30 strains of Hi_Ir isolated from the microbiology laboratory of the hospital were tested for drug sensitivity by disk diffusion method(K-B method)and Epsilometer test method(E-test method),β-lactamase detection and serotype identification.The hospital information system was used to retrieve relevant clinical data and analyze the clinical characteristics of the strain.Results The 30 Hi_Ir strains were isolated from 28 patients,including 21 males and 7 females,aged from 3 months to 85 years old,patients aged 0 to younger than 15 accounted for the highest proportion of 57.14%.All 30 Hi_Ir strains were isolated from respiratory tract specimens,of which 9 strains were pathogenic bacteria,14 strains were respiratory colonizing bacteria,and the remaining 7 strains were unknown bacteria.Other respiratory pathogenic microorganisms were also detected in 12 specimens.There were 15β-lactamase positive and negative strains respectively.The results of the two drug susceptibility test methods showed that 30 Hi_Ir strains were all sensitive to ceftriaxone and levofloxacin,K-B method detected 7 ampicillin-sensitive strains,K-B method detected that the Hi_Ir strains were all sensitive to meropenem,but E-test method detected 4 Hi_Ir strains resistant to meropenem.There was no statistically significant difference on the resistance rates of ampicillin,amoxicillin/clavulanic acid,and meropenem detected by the K-B method and the E-test method(P>0.05).Conclusion The Hi_Ir isolated from this hospital mainly causes respiratory infections and is still highly sensitive to ceftriaxone,levofloxacin,and meropenem.

关 键 词：流感嗜血杆菌 亚胺培南 耐药性 药敏试验 

分 类 号：R446.5[医药卫生—诊断学]