羊肚菌菌种继代培养代数与菌种退化关系  

Relationship Between the Subculture Times and Strain Degradation of Morchella importuna

作　　者：钱可晴 成盼盼 范君佩 徐安然 杨迪 张波[1] 李晓[1,3,4] QIAN Keqing;CHENG Panpan;FAN Junpei;XU Anran;YANG Di;ZHANG Bo;LI Xiao(Engineering Research Center of Chinese Ministry of Education for Edible and Medicinal Fungi,Jilin Agricultural University,Changchun 130118,China;School of Biology and Food Engineering,Huanghuai University,Zhumadian 463000,China;Key Laboratory of Edible Fungi Resources and Utilization(Northern),Ministry of Agriculture and Rural Areas,Jilin Agricultural University,Changchun 130118,China;Joint Laboratory of Modern Agricultural Technology International Cooperation,Ministry of Education,Jilin Agricultural University,Changchun 130118,China)

机构地区：[1]吉林农业大学食药用菌教育部工程研究中心,长春130118 [2]黄淮学院生物与食品工程学院,驻马店463000 [3]吉林农业大学农业农村部北方食用菌资源利用重点实验室,长春130118 [4]吉林农业大学现代农业技术教育部国际合作联合实验室,长春130118

出　　处：《菌物研究》2021年第4期277-284,共8页Journal of Fungal Research

基　　金：国家重点研发计划项目子课题(2019YFD1001905-33)。

摘　　要：为探究羊肚菌继代培养代数同菌种退化的关系,对梯棱羊肚菌菌丝体进行继代培养,并对各代菌丝显微形态、菌落形态、菌丝生长速度和胞外酶活性进行观察和测定,以揭示母种的频繁继代与菌种退化的关系。研究结果表明:随着继代培养代数的增加,菌丝生长速度、菌丝显微形态、菌落形态以及酶活性总体呈现下降趋势,要保持菌种的优良性状及活性,继代代数少于6代为宜。其中,继代1代(G1代)在22℃条件下,菌丝体的生长速度呈稳定上升趋势,菌丝浓密度均一,主干菌丝与侧生菌丝间的分叉约为45°,可以形成大量的菌核,且酶活力最强。In order to explore the relationship between subculture times and strain degradation of Morchella importuna,this study conducts the subculture on Morchella importuna and analyzes the growth rate,colony morphology,microstructure and extracellular enzyme activity of each generation of hyphae.It was found that with the increase of subculture times,the growth rate,growth force and enzyme activity of mycelia presented a downward trend.In order to maintain the excellent characteristics and the activity of the strain,the subculture times should be less than 6.Under 22℃,the mycelium(G1)growth showed a trend of steady rise,uniform mycelium density,main bifurcation between hypha and lateral with the angle of about 45°,a lot of sclerotium and strongest enzyme activity.

关 键 词：梯棱羊肚菌 继代 菌种退化 胞外酶活性 

分 类 号：Q949.325[生物学—植物学] S646[农业科学—蔬菜学]