柠檬桉油自微乳递送系统的制备及其药剂学性能  

Preparation of lemon eucalyptus oil self-micromilk delivery system and pharmaceutical properties

作　　者：兰斯琦 刘欢欢 支苑 张丽丹 唐永瑜 汪巳卜 张敏[2,3] 沈祥春 陶玲 LAN Siqi;LIU Huanhuan;ZHI Yuan;ZHANG Lidan;TANG Yongyu;WANG Sipu;ZHANG Min;SHEN Xiangchun;TAO Ling(School of Pharmacy,Guizhou Medical University,Guiyang 550025,Guizhou,China;Guizhou Engineering Center for Efficient Utilization of Natural Medicine Resources,Guizhou Provincial Key Laboratory of Pharmacology and Dedication Evaluation of Natural Medicine of Higher Education Institutions,Guizhou Medical University-Guiyang Joint Key Laboratory,Key Laboratory of Efficient Utilization of Natural Medicine Resources,Guiyang 550025,Guizhou,China;School of Basic Medicine,Guizhou Medical University,Guiyang 550025,Guizhou,China)

机构地区：[1]贵州医科大学药学院,贵州贵阳550025 [2]贵州省特色天然药物资源高效利用工程中心,贵州省高等学校天然药物药理与成药性评价特色重点实验室,贵州医科大学-贵阳市联合重点实验室,天然药物资源优效利用重点实验室,贵州贵阳550025 [3]贵州医科大学基础医学院,贵州贵阳550025

出　　处：《贵州医科大学学报》2022年第1期31-37,共7页Journal of Guizhou Medical University

基　　金：贵州省科技厅科技支撑计划(黔科合支撑[2017]2891);贵州省十百千人才项目(黔科合人才[2015]4029);贵州省国际科技合作基地(黔科合平台人才[2017]5802)。

摘　　要：目的探讨柠檬桉油自微乳递送系统(SMEDDS)的制备及其药剂学性能。方法通过改变处方配比,制备不同的柠檬桉油自微乳,绘制伪三元相图确定微乳液的有效自乳化区域;以自微乳平均粒径为响应值,采用星点设计-效应面法对柠檬桉油自微乳处方进行优化,筛选柠檬桉油自微乳处方中的乳化剂和助乳化剂并验证;取最佳处方制备所得的柠檬桉油自微乳超纯水稀释10倍,观察外观,采用马尔文激光电位粒度仪测粒径大小、分布情况及Zeta电位。结果柠檬桉油SMEDDS的最佳处方是柠檬桉油(45%)为油相、巴斯夫15-羟基硬脂酸聚乙二醇酯(HS15,36.7%)为乳化剂、无水乙醇(18.3%)为助乳化剂、乳化剂及助乳化剂比例为2∶1,滴加纯水9 mL,得外观呈半透明带蓝色乳光的柠檬桉油SMEDDS,激光粒径电位仪测得平均粒径为(49.05±0.33)nm,Zeta电位为(4.66±0.18)mV。结论柠檬桉油SMEDDS制备方法简便,重复性好,所得柠檬桉油SMEDDS外观良好,理化性质稳定。Objective To study the preparation and pharmacy properties of lemon eucalyptus oil self-microemulsion drug delivery system(SMEDDS).Method Different lemon eucalyptus oil self-microemulsion was prepared by changing the prescription ratio to determine the effective self-emulsification region of the microemulsion.Using the average particle size of self-micromilk as the response value,the star point design-effect surface method was optimized,and the emulsifier in the micromilk prescription were screened and validated.The lemon eucalyptus oil obtained from optimal prescription preparation was diluted 10 times from microemulsion ultra-pure water,and the appearance was observed.The particle size,distribution,and Zeta potential were measured by Malvin laser potential granometer.Result The optimal formula for SMEDDS of lemon eucalyptus oil was as follows:lemon eucalyptus oil(45%)as oil phase,HS15(36.7%)as emulsifier,anhydrous ethanol(18.3%)as co-emulsifier,and the ratio of emulsifier to co-emulsifier(Km)was 2∶1.After adding 9 mL pure water,the SMEDDS of lemon eucalyptus oil with a translucent blue opalescence was obtained.The results showed that the average particle size was(49.05±0.33)nm and the Zeta potential was(4.66±0.18)mV.Conclusion The preparation method of lemon eucalyptus oil SMEDDS is simple and reproducible,and has stable physical and chemical properties.

关 键 词：柠檬桉油 自微乳递送系统 伪三元相图 星点设计-效应面法:处方优化 生物利用度 

分 类 号：R94[医药卫生—药剂学] R283[医药卫生—药学]