某社区卫生服务中心医务人员咽拭子混采管标本SARS-CoV-2核酸阳性的溯源  

Tracing the source of SARS-CoV-2 nucleic acid positive in pharyngeal swab mixed collection tube samples of medical staff in a community health service center

作　　者：王共飞[1] 汪涛 查涛[1] 王毅[1] WANG Gong-fei;WANG Tao;ZHA Tao;WANG Yi(Wuhu Center for Disease Control and Prevention,Anhui 241000,China)

机构地区：[1]芜湖市疾病预防控制中心,安徽241000

出　　处：《安徽预防医学杂志》2021年第6期433-436,共4页Anhui Journal of Preventive Medicine

基　　金：芜湖市十四五医学重点专科(2021-2025);芜湖市卫生健康委科研项目(2021-123)。

摘　　要：目的快速查明2021年8月芜湖市某社区卫生服务中心医务人员咽拭子标本SARS-CoV-2核酸阳性原因,提升COVID-19精准防控能力。方法应用实时荧光RT-PCR技术,对某社区卫生服务中心医务人员10:1咽拭子混采管标本(某第三方检测机构SARS-CoV-2核酸初筛阳性)及重新采集的咽拭子单采管标本进行SARS-CoV-2核酸复核检测,核酸阳性标本进行SARS-CoV-2 D614G突变位点检测。随后采集该社区卫生服务中心外环境涂抹样进行SARS-CoV-2核酸污染状况评估检测。结果 3份10:1咽拭子混采管标本中2份核酸双靶标(ORF1ab+N)阳性,35份医务人员和医学实习生咽拭子单采管标本核酸双靶标阴性。混采管阳性标本未发生SARS-CoV-2 D614G位点变异,推断来源于SARS-CoV-2野生株(疫苗株)。12份外环境标本中3份单靶标阳性,其中1份ORF1ab基因阳性,来源于COVID-19疫苗接种人员工作服;2份N基因阳性,来源于COVID-19疫苗接种室键盘和普通疫苗接种室键盘鼠标。结论某社区卫生服务中心医务人员咽拭子混采管标本SARS-CoV-2核酸阳性是由COVID-19疫苗污染导致。Objective To quickly find out the cause of SARS-CoV-2 nucleic acid positive in pharyngeal swab samples of medical staff in a community health service center in Wuhu in August 2021,and improve the precision prevention and control ability of COVID-19.Methods Using real-time fluorescent RT-PCR technology, 10:1 pharyngeal swab mixed tube samples(SARS-CoV-2 nucleic acid screening positive by a third-party testing organization) and recollected pharyngeal swab single tube samples from a community health service center were rechecked for SARS-CoV-2 nucleic acid, and SARS-CoV-2 D614 G mutation sites were detected in nucleic acid positive samples.Then, smear samples from the environment outside the community health service center were collected for SARS-CoV-2 nucleic acid pollution assessment and detection.Results Two of the three 10:1 pharyngeal swab mixed collection tube specimens were positive for nucleic acid double target(ORF1 ab+N),and 35 pharyngeal swab single collection tube specimens of medical staff and medical interns were negative for nucleic acid double target.There was no variation of SARS-CoV-2 D614 G locus in the positive samples of mixed collection tubes, which was inferred to be from the wild strain of SARS-CoV-2(vaccine strain).Among the 12 environmental samples, 3 were single target positive, of which 1 was ORF1 ab gene positive, which came from the work clothes of COVID-19 vaccinators;Two N gene positive samples came from COVID-19 Vaccination Room keyboard and ordinary vaccination room keyboard and mouse.Conclusion The SARS-CoV-2 nucleic acid positive of pharyngeal swab mixed collection tube samples of medical staff in a community health service center was caused by COVID-19 vaccine contamination.

关 键 词：社区 医务人员 新冠病毒 新冠疫苗 接种场所环境 混采标本 

分 类 号：R181.8[医药卫生—流行病学]