SUMO化蛋白质组的富集策略研究进展  

Research Advances of Enrichment Approaches for SUMOylated Proteome

作　　者：李洋[1,2,3] 单亦初 梁振[1] 张丽华[1] 张玉奎[1] LI Yang;SHAN Yi-chu;LIANG Zhen;ZHANG Li-hua;ZHANG Yu-kui(Key Laboratory of Separation Science for Analytical Chemistry,Dalian Institute of Chemical Physics,Chinese Academy of Sciences,Dalian 116023,China;University of Chinese Academy of Sciences,Beijing 100049,China;Institute of Drug Discovery Technology,Ningbo University,Ningbo 315211,China)

机构地区：[1]中国科学院分离分析化学重点实验室中国科学院大连化学物理研究所,辽宁大连116023 [2]中国科学院大学,北京100049 [3]宁波大学新药技术研究院,浙江宁波315211

出　　处：《分析测试学报》2022年第1期58-62,共5页Journal of Instrumental Analysis

基　　金：国家重点研发计划项目(2017YFA0505003)。

摘　　要：蛋白质的SUMO(Small ubiquitin-like modifier)化修饰是生物体内一类重要的翻译后修饰,与核转运、转录调控、基因完整性维持和细胞周期调控等重要生物学过程密切相关。然而由于SUMO本身天然丰度低以及氨基酸序列冗长,SUMO化修饰的分析鉴定一直是研究的热点和难点。为实现SUMO化蛋白质组的深度覆盖分析,发展高效的SUMO化蛋白质/肽段的富集方法十分必要。该文对SUMO化蛋白质组不同富集方法的原理、特点以及最新研究进展进行了综述,并对其发展前景进行了展望。Protein SUMOylation is one of the most important post-translational modifications,which involved in various biological processes,including nuclear transport,transcriptional regulation,maintenance of gene integrity and cell cycle.However,identification of SUMOylation has always been the hotspot and difficulty in this field due to the low abundance and lengthy peptide sequence of endogenous SUMOylation.Therefore,the efficient enrichment of SUMOylated proteins/peptides is necessary to achieve deep coverage analysis of SUMOylome.The basic principles,characteristics and latest progresses of different enrichment methods for SUMOylated proteome are reviewed,and the development prospects are prospected.

关 键 词：SUMO化修饰 深度覆盖 富集 检测 

分 类 号：O629.7[理学—有机化学] Q493.2[理学—化学]