银黄解毒消肿胶囊对急性咽炎模型鼠的抗炎止痛作用  

Anti-Inflammatory and Analgesic Effects of Yinhuang Jiedu Xiaozhong Capsules on Acute Pharyngitis in Rats

作　　者：黄莉[1] 王兴 罗黎霞 杨宁 贺灿 陈可 谢嘉驰 罗纯清 HUANG Li;WANG Xing;LUO Lixia;YANG Ning;HE Can;CHEN Ke;XIE Jiachi;LUO Chunqing(The First Hospital of Hunan University of Chinese Medicine,Changsha,Hunan,China 410007;Xiangtan Chinese Medicine Hospital,Xiangtan,Hunan,China 411100)

机构地区：[1]湖南中医药大学第一附属医院,湖南长沙410007 [2]湖南省湘潭市中医医院,湖南湘潭411100

出　　处：《中国药业》2022年第2期32-35,共4页China Pharmaceuticals

基　　金：湖南省中医药科研计划项目[201681];湖南省“十三五”中医重点专科建设项目[湘中医药函[2017]86号];湖南省老中医药专家学术经验继承项目[湘中医药函[2020]37号];湖南省湘潭市指导性科技计划项目[ZDX-SF2019015];湖南中医药大学校级科研基金[2020XJJJ044]。

摘　　要：目的探讨银黄解毒消肿胶囊对急性咽炎模型鼠的抗炎及止痛作用。方法将60只SD大鼠随机分为空白对照组(A组,等体积0.9%氯化钠溶液),模型组(B_(1)组,等体积0.9%氯化钠溶液),阳性药物组(C_(1)组,咽炎含片1.1 g/kg)及银黄解毒消肿胶囊低、中、高剂量组(D_(1)组、D_(2)组、D_(3)组,2.02,4.04,8.08 g/kg),各10只。用喉头喷雾器向大鼠喷25%氨水以复制急性咽炎大鼠模型,建模成功后,各组大鼠咽部喷雾相应药物或灌胃0.9%氯化钠溶液,每日1次,连续7 d。采用酶联免疫吸附(ELISA)法检测大鼠血清白细胞介素1β、肿瘤坏死因子-α、前列腺素E;水平,观察大鼠咽部黏膜病变情况,并进行病理组织形态学评分。将50只雌性ICR小鼠随机分为模型组(B_(2)组,等体积0.9%氯化钠溶液),阳性药物组(C_(2)组,罗通定片0.02 g/kg)及银黄解毒消肿胶囊低、中、高剂量组(D_(4)组、D_(5)组、D_(6)组,2.92,5.84,11.68 g/kg),各10只,分别灌胃相应药物或0.9%氯化钠溶液,每日1次,连续3 d,采用热板实验测定痛阈值;另取50只雌性ICR小鼠,同热板实验分组及给药(记为B_(3),C_(3),D_(7),D_(8),D_(9)组),采用扭体实验记录扭体次数。结果与B1组比较,C_(1)组、D_(1)组、D_(2)组、D_(3)组大鼠白细胞计数、中性粒细胞计数,以及白细胞介素1β、肿瘤坏死因子-α、前列腺素E_(2)水平均显著降低(P<0.01),且咽部黏膜上皮层增生,固有层水肿,小血管扩张、充血,炎性细胞浸润情况均明显改善,病理组织形态学评分明显降低(P<0.01);与B_(2)组比较,C_(2)组、D_(4)组、D_(5)组、D_(6)组小鼠给药后30 min起痛阈值明显升高(P<0.01);与B_(3)组比较,C_(3)组、D_(3)组、D_(7)组、D_(8)组、D_(9)组小鼠扭体次数明显减少(P<0.01)。结论银黄解毒消肿胶囊能缓解急性咽炎模型鼠的炎性反应程度及疼痛,其抗炎机制可能与下调血清IL-1β,TNF-α,PGE_(2)水平有关。Objective To investigate the anti-inflammatory and analgesic effects of Yinhuang Jiedu Xiaozhong Capsules on acute pharyngitis in rats.Methods A total of 60 SD rats were randomly divided into blank control group(group A,equal volume of 0.9%Sodium Chloride Solution),model group(group B_(1),equal volume of 0.9%Sodium Chloride Solution),positive drug group(group C_(1),1.1 g/kg of Yanyan Buccal Tablets),and Yinhuang Jiedu Xiaozhong Capsules low-,medium-and high-dose groups(groups D_(1),D_(2) and D_(3),2.02,4.04,8.08 g/kg),with 10 rats in each group.The rat model of acute pharyngitis was established by spraying 25%ammonia with a throat spray.After successful modeling,rats in each group were sprayed with corresponding drugs or gavaged with 0.9%Sodium Chloride Solution once a day for 7 d.The levels of serum interleukin-1β(IL-1β),tumor necrosis factor-α(TNF-α)and prostaglandin E_(2)(PGE_(2))were detected by the enzyme-linked immunosorbent assay(ELISA).The pathological changes of pharyngeal mucosa of rats were observed and pathomorphological scores were performed.A total of 50 female ICR mice were randomly divided into model group(group B_(2),equal volume of 0.9%Sodium Chloride Solution),positive drug group(group C_(2),0.02 g/kg of Rotundine Tablets),and Yinhuang Jiedu Xiaozhong Capsules low-,medium-and high-dose groups(group D_(4),D_(5) and D_(6),2.92,5.84,11.68 g/kg),with 10 rats in each group,they were given corresponding drugs or 0.9%sodium Chloride Solution once a day for 3 d.The pain threshold was measured by the hot plate test.Another 50 female ICR mice were grouped and administered with hot plate test groups(groups B_(3),C_(3),D_(7),D_(8) and D_(9)),the inhibition rate of writhing reaction was calculated by the writhing test.Results Compared with those in group B_(1),the white blood cell count(WBC),neutrophil count,the levels of IL-1β,TNF-αand PGE_(2) in groups C_(1),D_(1),D_(2) and D_(3) decreased significantly(P<0.01),the pharyngeal mucosal epithelium proliferated,lamina propria edema,small vessel dilation

关 键 词：银黄解毒消肿胶囊 急性咽炎 大鼠 小鼠 抗炎 镇痛 作用机制 

分 类 号：R932[医药卫生—生药学] R285.5[医药卫生—药学]