藏药肺热普清散中乌头碱的限量检测  

Limitation Determination of Aconitine in Feire Puqing Powder

作　　者：马静 邹丽 王曙[2] MA Jing;ZOU Li;WANG Shu(Sichuan Institute of Drug Control·Sichuan Testing Center of Medicial Devices,Chengdu,Sichuan,China 611731;West China School of Pharmacy,Sichuan University,Chengdu,Sichuan,China 610041)

机构地区：[1]四川省药品检验研究院院·四川省医疗器械检测中心,四川成都611731 [2]四川大学华西药学院,四川成都610041

出　　处：《中国药业》2022年第2期68-70,共3页China Pharmaceuticals

摘　　要：目的建立测定藏药肺热普清散中乌头碱含量的高效液相色谱法。方法色谱柱为Phenomenex ODS柱(250 mm×4.6 mm,5μm),流动相为0.05 mol/L醋酸铵-乙腈(梯度洗脱),流速为1.0 mL/min,检测波长为235 nm,柱温为35℃,进样量为10μL。结果乌头碱进样量在0.046~1.440μg范围内与峰面积线性关系良好(r=0.999 6,n=6),检测限为1.2μg/mL;精密度、稳定性、重复性试验的RSD均小于2.0%(n=6),平均加样回收率为99.32%,RSD为0.41%(n=6)。结论所建立的方法稳定可靠,重复性好,可用于测定肺热普清散中乌头碱的含量。Objective To establish a high-performance liquid chromatography(HPLC) method for the determination of aconitine in Feire Puqing Powder.Methods The chromatographic column was Phenomenex ODS column(250 mm x 4.6 mm,5 μm),the mobile phase was 0.05 mol/L ammonium acetate-acetonitrile(gradient elution),the flow rate was 1.0 mL/min,the detection wavelength was 235 nm,the column temperature was 35℃,and the injection volume was 10 μL.Results The linear range of aconitine was 0.046-1.440 μg(r=0.999 6,n=6),and the limit of detection(LOD) was 1.2 μg/mL.The RSDs of precision,stability and repeatability tests were less than 2.0%(n=6),and the average recovery of aconitine was 99.32% with an RSD of 0.41%(n=6).Conclusion The method is stable,reliable and reproducible,which can used for the content determination of aconitine in Feire Puqing Powder.

关 键 词：乌头碱 肺热普清散 限量测定 高效液相色谱法 

分 类 号：R932[医药卫生—生药学] R284.1[医药卫生—药学]