凉血通瘀方对脑出血大鼠肠黏膜屏障修复及神经功能改善作用的研究  

Effect of Liangxue Tongyu Formula on Repairing Intestinal Mucosal Barrier and Improving Nerve Function in Rats with ICH

作　　者：张子健 李建香[3] 周雨 过伟峰[1] ZHANG Zijian;LI Jianxiang;ZHOU Yu;GUO Weifeng(Affiliated Hospital of Nanjing University of Chinese Medicine,Nanjing 210029,China;Key Laboratory for Metabolic Diseases in Chinese Medicine,Nanjing University of Chinese Medicine,Nanjing 210023,China;Nanjing Hospital of Traditional Chinese Medicine Affiliated to Nanjing University of Chinese Medicine,Nanjing 210022,China)

机构地区：[1]南京中医药大学附属医院,江苏南京210029 [2]南京中医药学大学代谢病中医研究重点实验室,江苏南京210023 [3]南京中医药大学附属南京中医院,江苏南京210022

出　　处：《中医药信息》2022年第1期29-34,共6页Information on Traditional Chinese Medicine

基　　金：国家自然科学基金项目(81704017);中央本级重大增减支项目(2060302);江苏省科技项目(BK20191410,BK20211011);江苏省南京市卫生科技发展专项资金项目(JQX18006)。

摘　　要：目的:探究凉血通瘀方对脑出血大鼠肠黏膜屏障的组成结构、选择通透性以及神经功能的影响。方法:将30只SD大鼠随机分为正常组、假手术组、模型组、凉血通瘀组和益生菌组。除正常组和假手术组外,其余各组大鼠通过注射泵控制器注血/二次退针法建立脑出血模型。造模成功后,凉血通瘀组给予凉血通瘀方药液灌胃,益生菌组给予双歧杆菌三联活菌制剂灌胃,每日1次,连续3 d。治疗结束后,采用ELISA法检测肠黏膜屏障蛋白Occludin、Claudin-1、ZO-1的表达水平及血清中LPS、DAO、DLA的含量。结果:与模型组比较,凉血通瘀组行为学评分显著下降(P<0.01),益生菌组评分改变不明显(P>0.05)。与正常组比较,模型组Occludin、Claudin-1、ZO-1蛋白的表达水平显著降低(P<0.01),血清LPS、DAO、DLA的含量显著增加(P<0.01);凉血通瘀组和益生菌组Claudin-1、ZO-1蛋白的表达水平明显降低(P<0.05),血清LPS、DAO、DLA的含量明显增加(P<0.05)。与假手术组比较,模型组Claudin-1、ZO-1蛋白的表达水平显著降低(P<0.01),血清LPS、DAO、DLA的含量显著增加(P<0.01),凉血通瘀组ZO-1蛋白的表达水平明显降低(P<0.05),血清LPS、DAO、DLA的含量显著增加(P<0.01);益生菌组血清LPS的含量显著升高(P<0.01)。与模型组比较,凉血通瘀组Claudin-1、ZO-1蛋白的表达水平明显上升(P<0.05),益生菌组Occludin、Claudin-1、ZO-1蛋白的表达水平均显著上升(P<0.01)。与模型组比较,凉血通瘀组与益生菌组血清LPS、DAO、DLA的含量显著降低(P<0.01)。与凉血通瘀组比较,益生菌组血清DLA的含量显著降低(P<0.01)。结论:凉血通瘀方与益生菌制剂均可有效提高肠黏膜屏障紧密连接蛋白Occludin、Claudin-1和ZO-1的表达水平,改善肠黏膜通透性,减少LPS、DAO及DLA等有害物质入血,且益生菌制剂稍优于凉血通瘀方。但在改善脑出血后的神经功能缺损症状方面,凉血通瘀方疗效更为确切。Objective:To investigate the effects of Liangxue Tongyu Formula on intestinal mucosal barrier structure,selective permeability and nerve function in rats with intracerebral hemorrhage(ICH).Methods:30 SD rats were randomly divided into the normal group,the sham operation group,the model group,the Liangxue Tongyu group and the probiotic group.ICH model was made by blood injection/secondary needle withdrawal by the injection pump controller.After the model was successfully established,the Liangxue Tongyu group and the probiotic group were given Liangxue Tongyu Formula and probiotic preparation by gavage respectively.The expression levels of intestinal mucosal barrier proteins of Occludin,Claudin-1 and ZO-1,as well as the serum contents of LPS,DAO DLA were detected by ELISA.Results:In terms of the behavioral scores,they were significantly decreased in the Liangxue Tongyu group compared to those in the model group(P<0.01);but there was no statistical difference between the model group and the probiotic group(P>0.05).Compared to those in the normal group,the protein expressions of Occludin,Claudin-1 and ZO-1 were significantly decreased in the model group(P<0.01),and the serum contents of LPS,DAO and DLA were significantly increased in the model group(P<0.01);the protein expressions of Claudin-1 and ZO-1 were significantly decreased(P<0.05),and the serum contents of LPS,DAO and DLA were significantly increased in the Liangxue Tongyu group and in the probiotics group(P<0.05).Compared to those in the sham operation group,the protein expressions of Claudin-1 and ZO-1 were significantly decreased(P<0.01),and the serum contents of LPS,DAO and DLA were significantly increased in the model group(P<0.01);the protein expression of ZO-1 was significantly decreased(P<0.05),and the serum contents of LPS,DAO and DLA were significantly increased in the Liangxue Tongyu group(P<0.01);the serum content of LPS was significantly increased in the probiotics group(P<0.01).Compared to those in the model group,the protein expressions of Clau

关 键 词：脑出血 凉血通瘀方 益生菌 肠黏膜屏障 紧密连接蛋白 脂多糖 二胺氧化酶 D-乳酸 

分 类 号：R285.5[医药卫生—中药学]