蒺藜苜蓿cation/H+exchanger基因家族鉴定及表达特征分析  

Identification and expression characteristics of a cation/H+exchanger gene family in Medicago truncatula

作　　者：吴彤 刘云苗 金军 董伟峰 才晓溪 孙明哲[1] 贾博为 孙晓丽[1] WU Tong;LIU Yun-miao;JIN Jun;DONG Wei-feng;CAI Xiao-xi;SUN Ming-zhe;JIA Bo-wei;SUN Xiao-li(Crop Stress Molecular Biology Lab,Heilongjiang Bayi Agricultural University,Daqing 163319,China)

机构地区：[1]黑龙江八一农垦大学作物逆境分子生物学实验室,黑龙江大庆163319

出　　处：《草业学报》2022年第1期181-194,共14页Acta Prataculturae Sinica

基　　金：黑龙江八一农垦大学大学生创新创业训练计划项目(202010223045);国家自然科学基金(32000212);中央支持地方高校改革发展资金人才培养支持计划项目,黑龙江八一农垦大学基础培育计划(ZRCPY201902);黑龙江八一农垦大学引进人才科研启动计划(XYB201902)项目资助。

摘　　要：CPA(cation proton antiporter)超家族通过转运质子和一价金属离子调节细胞内离子和pH稳定。阳离子质子转运体(cation/H^(+)exchanger,CHX)基因家族属于CPA2(cation proton antiporter 2)超家族,其N端有一个Na^(+)/H^(+)exchanger结构域,对植物维持细胞离子平衡、花器官发育起着至关重要的作用。通过生物信息学手段,系统地分析了蒺藜苜蓿CHX家族基因,通过全基因组筛选共鉴定出47个MtCHXs;染色体定位分析表明蒺藜苜蓿CHX基因分布在8条不同的染色体上;同源性分析显示蒺藜苜蓿和拟南芥亲缘关系近,而与水稻亲缘关系远;进一步进化关系分析将47个MtCHXs基因分为5组,并且各组内成员在基因结构和基序上比较保守;顺式作用元件分析发现MtCHXs基因启动子包含大量光响应元件、激素响应元件以及干旱、低温、创伤响应元件;表达特性分析发现MtCHXs在生殖器官中高表达,并且响应干旱、低温等非生物胁迫。The CPA(cation proton antiporter)superfamily regulates intracellular ion and pH homeostasis by transporting proton and monovalent metal ions.Cation/H^(+)exchangers(CHX)belong to cation proton antiporter 2(CPA2)superfamily.CPA2 members contain an Na^(+)/H^(+)exchanger domain at the N terminus,which plays an important role in maintaining ion balance and flower organ development.In this study,CHX family genes from Medicago truncatula were systematically analyzed using bioinformatics,and a total of 47 non-redundant MtCHXs were identified by genome-wide screening.Chromosome location analysis showed that MtCHXs were distributed on 8 different chromosomes.Homology analysis indicated that alfalfa was closely related to Arabidopsis,but far removed from rice(Oryza sativa).According to their evolutionary relationship,the 47 MtCHXs were further divided into 5 groups,and gene structure and motif of MtCHX genes within each group were conserved.In addition,analysis of the cis-acting element revealed that the MtCHXs gene promoter contained numbers of light-responsive elements,hormone-responsive elements,drought,low temperature and wound responsive elements.Further analysis of expression characteristics indicated that MtCHXs were highly expressed in reproductive organs and responded to abiotic stresses such as drought and low temperature.

关 键 词：蒺藜苜蓿 阳离子质子转运体 CHX 生物信息学 

分 类 号：S541.9[农业科学—作物学]