不同药剂诱导玉米无融合生殖快速获得二倍体技术研究  

Study on Diploid Aquirement of Maize by Apomixis Induced by Different Medicaments

作　　者：姚威港 王晓东[1] 李志刚[1] 史铎 刘鹏[1] YAO Weigang;WANG Xiaodong;LI Zhigang;SHI Duo;LIU Peng(Inner Mongolia University For Nationalities,Liaotong Inner Mongolia 028000,China)

机构地区：[1]内蒙古民族大学农学院,内蒙古通辽028000

出　　处：《种子》2021年第12期144-148,共5页Seed

基　　金：内蒙古民族大学博士科研启动基金项目(BS 525)。

摘　　要：采用氟乐灵、聚乙烯亚胺、秋水仙素、戊炔草胺和青鲜素5种药剂,设置20、40、80、160、320μmol·L^(-1)等5种不同浓度及不同处理时期,分别对糯玉米和普通玉米进行无融合生殖的诱导。结果表明,当试剂浓度为80μmol·L^(-1)时诱导效果最佳;最佳诱导时期为吐丝后第5天,结实株率明显高于其他时期,平均高出6%~10%;糯玉米对玉米孤雌诱导的影响大于普通玉米;本试验还筛选出聚乙烯亚胺等毒性较低的新型诱导剂代替秋水仙素,并有效提高了诱导率。In this paper, the induction of apomixis of waxy corn and common corn was carried out with fluuralin, polyvinyl imide, colchicine, pentyleamine and cyanocyanin at five concentrations including 20,40,80,160,320 μmol·L^(-1) at different treatment periods.The results showed that the best induction effect was obtained when the reagent concentration was 80 μmol·L^(-1),and the best induction period was the 5 th day after silking and the seed rate was 6%-10% higher than other periods.Also, the effect of waxy corn on maize parthenogenesis induction was greater than that of common corn.Additionally, new inducers with lower toxicity such as polyethylenimine were selected to replace colchicine and the induction rate was improved effectively.

关 键 词：玉米 化学试剂 无融合生殖 诱导技术 

分 类 号：S513[农业科学—作物学]