傣药清风颗粒质量标准的研究  

Study on quality standard of Daiyao Qingfeng Granules

作　　者：刘思雨 敬应春 苏靖 郑博 郑梦园 邱明丰[1] LIU Siyu;JING Yingchun;SU Jing;ZHENG Bo;ZHENG Mengyuan;QIU Mingfeng(School of Pharmacy,Shanghai Jiao Tong University,Shanghai 200240,China;Guizhou Sinorda Biomedicine Co.,Ltd.,Guiyang 550009,China)

机构地区：[1]上海交通大学药学院,上海200240 [2]贵州生诺生物科技有限公司,贵州贵阳550009

出　　处：《沈阳药科大学学报》2021年第12期1319-1326,共8页Journal of Shenyang Pharmaceutical University

基　　金：上海市科委项目(17401900900)。

摘　　要：目的建立傣药清风颗粒(土茯苓、杧果叶、肾茶、黄芪、延胡索、藤茶、北沙参、傣草蔻根、墨旱莲)的质量标准。方法薄层色谱法(TLC)定性鉴别土茯苓、杧果叶、肾茶、黄芪、延胡索、藤茶、傣草蔻根、墨旱莲。高效液相色谱法(HPLC)测定落新妇苷、芒果苷、迷迭香酸含量。结果TLC斑点清晰,阴性无干扰。落新妇苷、芒果苷、迷迭香酸分别在19.97~99.84、19.63~98.17、2.25~11.25 mg·L^(-1),范围内线性关系良好(r=0.999),平均加样回收率分别为99.12%、96.92%、98.06%(RSD<3%)。结论该方法准确可靠,重复性好,专属性强,可用于傣药清风颗粒的质量控制。Objective To establish the quality standard of Daiyao Qingfeng Granules.Methods TLC was used to identify Smilacis Glabrae Rhizoma,Spicate Clerodendranthus,Mangiferae folium,Astragali Radix,Corydalis Rhizoma,Ampelopsis Cantoniensis Caulis,Ecliptae Herba and Alpineae Kwangsiensis Rhizoma.HPLC was used to determine the contents of Astilbin,mangiferin and rosmarinic acid.Results The TLC spots were clear without negative interference.Astilbin,mangiferin and rosmarinic acid showed good linear relationships in the ranges of 19.97-99.84,19.63-98.17,2.25-11.25 mg·L^(-1)(r=0.999),whose average recoveries were 99.12%,96.92%,98.06% respectively(RSD<3%).Conclusion The method is simple,accurate and reliable.It can be used for the quality control of Daiyao Qingfeng Granules.

关 键 词：傣药清风颗粒 质量标准 TLC HPLC 

分 类 号：R917[医药卫生—药物分析学]