接种湿地底泥的Anammox反应器启动特性  

Start up characteristics of Anammox reactor inoculated with wetland sediment

作　　者：樊沈毅 程子波[1,3] 肖付耀 吴昊 邹华 FAN Shenyi;CHENG Zibo;XIAO Fuyao;WU Hao;ZOU Hua(School of Environmental and Civil Engineering,Jiangnan University,Wuxi 214122,China;Jiangsu Key Laboratory of Anaerobic Biotechnology,Wuxi 214122,China;Co.Ltd.of Suzhou Ruicheng Environmental Technology,Suzhou 215500,China;Jiangsu Collaborative Innovation Center of Technology and Material of Water Treatment,Suzhou University of Science and Technology,Suzhou 215009,China)

机构地区：[1]江南大学环境与土木工程学院,无锡214122 [2]江苏省厌氧生物技术重点实验室,无锡214122 [3]苏州锐诚环保技术有限公司,苏州215500 [4]苏州科技大学,江苏省水处理技术与材料协同创新中心,苏州215009

出　　处：《环境工程学报》2021年第11期3718-3728,共11页Chinese Journal of Environmental Engineering

基　　金：国家自然科学基金资助项目(51808260);江苏省自然科学基金资助项目(BK20180632);江苏省厌氧生物技术重点实验室开放基金资助项目(JKLAB201710)。

摘　　要：在3台UASB反应器中分别以厌氧颗粒污泥、湿地底泥以及二者的混合物(污泥浓度比为1∶1)为接种污泥启动厌氧氨氧化(Anaerobicammoniumoxidation,Anammox)反应器,考察了以湿地底泥为接种物启动Anammox反应器的可行性。结果表明,接种厌氧颗粒污泥的反应器经过183 d启动运行,仍未出现明显厌氧氨氧化反应特性,接种混合污泥的反应器于第125天开始出现明显厌氧氨氧化反应特性,短于接种湿地底泥的155 d。Anammox反应器启动并稳定运行后,在进水总氮质量浓度为275 mg·L^(-1)、负荷为0.275 kg·(m^(3)·d)^(-1)条件下,出水总氮质量浓度可降至90 mg·L^(-1)以下,其中接种湿地底泥和混合污泥的Anammox反应器的TN去除率分别达到74.49%和67.12%。高通量结果表明,在接种湿地底泥和混合污泥的反应器中的厌氧氨氧化菌属为Candidatus Brocadia,其丰度分别达到9.82%和10.70%。Anaerobic granular sludge,wetland sediment and their mixture(SS ratio of 1∶1)were used as inoculated sludge to start the Anammox reactor in three UASB tanks,and the feasibility of starting Anammox reactor with wetland sediment as inoculum was investigated.The results showed that the USAB inoculated with anaerobic granular sludge didn’t present the obvious Anammox reaction characteristics after 183 days of start-up operation.The USAB inoculated with mixed sludge began to show obvious Anammox reaction characteristics on day 125,which was shorter than USAB inoculated with wetland sediment on day 155.After the start-up and stable operation of the Anammox reactor,the total nitrogen concentration in the effluent could be reduced to less than 90 mg·L^(-1) under the conditions of the total nitrogen concentration in the influent of 275 mg·L^(-1) and the load of 0.275 kg·(m^(3)·d)^(-1).The TN removal rates of the Anammox reactor inoculated with wetland sediments and mixed sludge reached 74.49%and 67.12%,respectively.The high-throughput results showed that the Anammox bacteria in the two reactors were Candidatus Brocadia,and their abundances reached 9.82%and 10.70%,respectively.

关 键 词：厌氧氨氧化 湿地底泥 启动过程 微生物群落 

分 类 号：X703.1[环境科学与工程—环境工程]