常压室温等离子体诱变选育丁烯基多杀菌素高产菌株及培养基优化  

Breeding of Butenyl-spinosyns Producing Strain by Atmospheric Room Temperature Plasma and Optimization of Culture Medium

作　　者：张逍遥 郭超 王靖楠 刘艳丽[2] 王超 ZHANG Xiao-yao;GUO Chao;WANG Jing-nan;LIU Yan-li;WANG Chao(Academy of National Food and Strategic Reserves Administration,Beijing 100037,China;MCMM Center,Beijing City University,Beijing 100083,China)

机构地区：[1]国家粮食和物资储备局科学研究院,北京100037 [2]北京城市学院MCMM中心,北京100083

出　　处：《粮油食品科技》2022年第1期174-181,共8页Science and Technology of Cereals,Oils and Foods

基　　金：粮油食品储藏运输流通环节危害物综合防控技术研究(2017YFC1600604);中央级公益性科研院所基本科研业务费专项(JY2021)。

摘　　要：以ASAGF 2-G4为出发菌株,进行常压室温等离子体与2 mg/mL亚硝基胍的复合诱变选育。结合96孔板发酵培养进行了初筛及摇瓶复筛后,共得到高产突变菌株5株,经复筛验证获得1株高产且稳定遗传菌株2-A9,其比出发菌株产量提高30.92%。利用Pulckett-Burman实验设计筛选发酵培养基中的影响因素,得到葡萄糖、糊精、棉籽蛋白、玉米浆干粉为显著因素。利用Box-Behnken响应面实验设计优化4种影响因素,获得最优培养基配方为(g/L):葡萄糖60.0、糊精25.0、棉籽蛋白25.0、玉米浆干粉12.5、胨化牛奶10.0、Mg SO_(4) 1.0、NaCl 2.0、CaCO_(3) 5.0。经重复实验验证后,丁烯基多杀菌素平均产量与预测产量接近,表明该模型可行有效。The original strain ASAGF 2-G4 was applied to undergo Atmospheric Room Temperature Plasma(ARTP) mutagenesis of various doses and 2 mg/mL NTG compound mutagenesis of different action durations.Five high-yield mutant strains were obtained after preliminary screening through 96-orifice-plate fermentation and re-screening in shake flasks. At last, a high-yielding mutant strain 2-A9 was selected, which increased the yield by 30.92% compared with the original strain after genetic stability verification. A high-yield mutant ASAGF 2-A9 was applied as the starting strain, the experiment design Plackett-Burman was used to screen the factors affecting the fermentation media, with a conclusion that factor glucose, dextrin, cottonseed protein and corn starch is a significant factor affecting butenyl-spinosyns. The experimental design Box-Behnken was applied to optimize the factors significantly affecting the experiment Plackett-Burman. The optimal growing media components were deduced as follows(g/L): glucose 60.0, dextrin 25.0, cottonseed protein 25.0, coen steep dry powder 12.5, peptone milk 10.0, MgSO_(4) 1.0, NaCl 2.0, CaCO_(3) 5.0. It’s verified that the average output of butenyl-spinosyns is close to the predicted value, indicating that the model is feasible and effective.

关 键 词：丁烯基多杀菌素 常压室温等离子体 亚硝基胍 诱变 培养基优化 

分 类 号：S482.39[农业科学—农药学]