祛瘀化痰通脉方对高脂血症金黄地鼠肠道菌群的调节作用  

Regulatory Effect of Quyu Huatan Tongmai Prescription on Intestinal Microflora in Golden Hamster with Hyperlipidemia

作　　者：苗兰[1] 彭勍[1] 孙明谦[1] 张业昊 张颖[1] 任常英 刘建勋[1] 林力[1] MIAO Lan;PENG Qing;SUN Ming-qian;ZHANG Ye-hao;ZHANG Ying;REN Chang-ying;LIU Jian-xun;LIN Li(Institute of Basic Medical Sciences of Xiyuan Hospital,China Academy of Chinese Medical Sciences,Beijing Key Laboratory of Pharmacology of Traditional Chinese Medicine,Beijing 100091,China)

机构地区：[1]中国中医科学院西苑医院基础医学研究所,中药药理北京市重点实验室,北京100091

出　　处：《中国实验方剂学杂志》2022年第1期109-120,共12页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：中国中医科学院科技创新工程项目(C12021A04508);国家自然科学基金项目(81673587,81803770)。

摘　　要：目的:探究中药复方祛瘀化痰通脉方对高脂血症金黄地鼠肠道菌群的调节作用及功效配伍的科学性。方法:实验选用叙利亚金黄地鼠,分为正常、模型、复方、祛瘀、化痰和解毒组6组,每组8只。采用高脂饲料喂饲法形成金黄地鼠高脂血症模型,造模4周,灌胃给予祛瘀组(1.11 g·kg^(-1)),化痰组(0.39 g·kg^(-1)),解毒组(0.07 g·kg^(-1)),祛瘀化痰通脉方组(1.42 g·kg^(-1)),正常组与模型组给予等体积的蒸馏水,每天1次,连续灌胃6周。收集血清测定血脂,苏木素-伊红(HE)染色观察肝脏病理形态,收集粪便进行肠道菌群16S rRNA基因高通量测序分析。结果:与正常组比较,模型组金黄地鼠体质量增长明显(P<0.05,P<0.01),各项血脂指标均显著升高(P<0.01),肠道菌群多样性明显降低(P<0.05,P<0.01),菌群相对丰度门、科、属水平均发生明显变化(P<0.05,P<0.01)。与模型组比较,祛瘀化痰通脉方能控制高脂血症金黄地鼠体质量变化,降低血清甘油三酯(TG),总胆固醇(TC),低密度脂蛋白胆固醇/高密度脂蛋白胆固醇比值(LDL-C/HDL-C)(P<0.05,P<0.01);改善肠道菌群结构,显著降低厚壁菌门与拟杆菌门的比值(P<0.01),增加拟杆菌科、紫单孢菌科、理研菌科、巴氏杆菌属等菌群丰度(P<0.05,P<0.01),降低红蝽菌属的相对丰度(P<0.05)。各配伍组对高脂血症金黄地鼠血脂、肠道菌群具有不同程度的改善作用,其中解毒组降血脂作用和化痰组菌群调节作用更接近复方组。结论:祛瘀化痰通脉方及配伍组从不同角度对金黄地鼠高脂血症发挥不同程度的改善作用,其作用机制可能与调节肠道菌群结构、改善肠道微生态有关,复方组作用最为显著,化痰组次之,本研究亦为中药复方祛瘀化痰通脉方配伍的科学性提供了依据。Objective: To explore the regulatory effect of Quyu Huatan Tongmai prescription on intestinal mircoflora of hyperlipidemia golden hamster and scientific evidence for the compatibility. Method:Syrian golden hamsters were randomized into normal,model,prescription,stasis-dispelling(Quyu),phlegmdissolving(Huatan),and detoxification(Jiedu)groups,with 8 in each group. Hyperlipidemia in golden hamsters was induced by high-fat diet(4 weeks). Then hamsters in the Quyu group(1.11 g·kg^(-1)),Huatan group(0.39 g·kg^(-1)),Jiedu group(0.07 g·kg^(-1)),and prescription group(1.42 g·kg^(-1))were given(ig)corresponding drugs and those in the normal group and the model group received(ig)distilled water of equivalent volume,once a day for 6 weeks. Serum lipids were determined,and hematoxylin-eosin(HE)staining was used to observe the pathological morphology of the liver. Feces were collected for 16 S rRNA gene high-throughput sequencing of intestinal flora. Result: Compared with normal group,the model group demonstrated increase in body weight(P<0.05,P<0.01)and blood lipids(P<0.01),decrease in intestinal flora diversity(P<0.05,P<0.01),and variation of the relative abundance of intestinal flora at phylum,family,and genus levels(P<0.05,P<0.01). Compared with the model group,Quyu Huatan Tongmai prescription controlled the body weight change,reduced the serum triglyceride(TG),total cholesterol(TC),and low density lipoprotein cholesterol/high density lipoprotein cholesterol ratio(LDL-C/HDL-C)(P<0.05, P<0.01), improved the structure of intestinal flora, decreased the ratio of Firmicutes to Bacteroides(P<0.01), raised the abundance of Bacteroidaceae,Porphyromonadaceae,Rikenellaceae,and Pasteurella(P<0.05,P<0.01),and lowered the relative abundance of Coriobacterium(P<0.05)in hyperlipidemia golden hamsters. All the split prescriptions improved blood lipids and intestinal flora of the hamsters and particularly,the lipids-lowering effect of the Jiedu group and the regulation of flora in the Huatan group were closer to those of the prescript

关 键 词：祛瘀化痰通脉方 高脂血症 肠道菌群 复方配伍 

分 类 号：R2-0[医药卫生—中医学] R22