血浆miR-101水平与COPD急性加重期并呼吸衰竭患者气道反应性的关系探讨  

Relationship between Plasma miR-101 Level and Airway Reactivity in Patients with Acute Exacerbation of COPD and Respiratory Failure

作　　者：杜发强 DU Faqiang(The People’s Hospital of Zhoukou City,He’nan Province 466000)

机构地区：[1]河南省周口市人民医院,466000

出　　处：《医学理论与实践》2022年第2期198-201,共4页The Journal of Medical Theory and Practice

摘　　要：目的:探讨血浆微小核糖核酸-101(miR-101)水平与慢性阻塞性肺疾病(COPD)急性加重期并呼吸衰竭患者气道反应性的关系。方法:选取2017年2月—2019年3月本院收治的186例COPD急性加重期并呼吸衰竭患者进行研究,逆转录—聚合酶链反应法(RT-PCR)检测所有研究对象血浆miR-101水平。利用脉冲振荡肺功能测定气道高反应性;Logistic回归分析探讨血浆miR-101与气道高反应性的关系;制作受试者工作特征曲线(ROC),分析血浆miR-101水平对气道高反应性是否发生的评估效能。结果:本研究中有68例发生气道高反应性,发生率为36.56%;气道高反应性组患者血浆miR-101表达水平明显高于气道正常组(P<0.05)。气道高反应性组吸烟、COPD病程>5年、遗传因素、呼吸道感染、气道炎症、血浆miR-101水平>1.0构成比均显著高于气道正常组(P<0.05);2组患者肺功能损伤程度分布差异显著(P<0.05),气道高反应组肺功能重度损伤、肺功能极重度损伤构成比均显著高于气道正常组(P<0.05);经Logistic回归分析可知,吸烟、COPD病程>5年、遗传、呼吸道感染、气道炎症、肺功能重度损伤、肺功能极重度损伤、血浆miR-101>1.0均是影响气道高反应发生的独立危险因素(P<0.05)。受试者工作特征曲线(ROC)分析显示,血浆miR-101评估发生气道高反应性的最佳截断点为0.81,灵敏度为86.76%,特异度为73.73%,准确度为78.49%,曲线下面积(AUC)为0.851。结论:血浆miR-101在气道高反应患者中呈高表达,可用于评估COPD急性加重期并呼吸衰竭患者是否发生气道高反应性。Objective:To investigate the relationship between the plasma micro ribonucleic acid-101(miR-101)level and the airway reactivity in patients with acute exacerbation of COPD and respiratory failure.Methods:186 patients with acute exacerbation of COPD and respiratory failure admitted to our hospital from February 2017 to March 2019 were selected for study.The plasma miR-101 level of all subjects was detected by Reverse transcription polymerase chain reaction(RT-PCR).Measurement of airway hyperresponsiveness by impulse oscillation pulmonary function.The relationship between plasma miR-101 and airway hyperresponsiveness by logistic regression analysis.The receiver operating characteristic curve(ROC)of subjects was made to analyze the evaluation effect of plasma miR-101 level on the occurrence of airway hyperresponsiveness.Results:There were 68 cases of airway hyperresponsiveness in this study,the incidence was 36.56%.The expression level of miR-101 in patients with airway hyperresponsiveness group was significantly higher than that in patients with normal airway group(P<0.05).The proportion of smoking,COPD course>5 years,genetic factors,respiratory tract infection,airway inflammation and miR-101 level>1.0 in the airway hyperresponsiveness group was significantly higher than that in the normal airway group(P<0.05).There was a significant difference in the degree of lung function injury between the two groups(P<0.05),and the proportion of severe injury and extremely severe injury in the airway hyperresponsiveness group were significantly higher than those in normal airway group(P<0.05).According to logistic regression analysis,smoking,COPD course>5 years,heredity,respiratory tract infection,airway inflammation,severe injury of lung function,extremely severe injury of lung function,and plasma miR-101>1.0 were all independent risk factors(P<0.05).Receiver operating characteristic curve(ROC)analysis showed that the best cutoff point of miR-101 was 0.81,the sensitivity was 86.76%,the specificity was 73.73%,the accuracy was

关 键 词：miR-101 COPD 急性加重期 呼吸衰竭 气道反应性 

分 类 号：R562[医药卫生—呼吸系统] R392[医药卫生—内科学]