机构地区:[1]西南大学柑橘研究所/国家柑橘工程技术研究中心,重庆400712 [2]重庆文理学院化学与环境工程学院/环境材料与修复技术重庆市重点实验室,重庆402160
出 处:《浙江农业学报》2022年第1期89-97,共9页Acta Agriculturae Zhejiangensis
基 金:重庆市技术创新与应用发展专项(cstc2019jscx-gksbX0102);财政部和农业农村部:国家现代农业产业技术体系;中央高校基本业务费专项资金(XDJK2019B041)。
摘 要:为阐明不同发光二极管(LED)光质对金秋砂糖橘生长发育和光合特性的影响,以金秋砂糖橘容器苗为试验材料,设置LED红光(R)、蓝光(B)、红蓝复合光(RB11、RB21、RB41、RB81)共6种光质处理,并以LED白光(W)为对照组。处理2个月后研究新梢茎叶形态、生物量、光合色素含量和光合特征参数变化,并对相关指标进行主成分分析。结果表明:红光显著促进新梢茎长、叶片数、叶面积和生物量,抑制茎粗和叶厚,而蓝光的作用与红光相反;低比例红光显著促进新梢茎长、茎粗、叶片数、叶面积和生物量,但随着红光比例的增加呈显著降低趋势,其中,RB11处理的茎长、茎粗、叶片数和叶面积分别是对照组的1.97倍(P<0.05)、1.14倍(P>0.05)、2.05倍(P<0.05)、1.84倍(P<0.05),新梢鲜重和干重,以及叶鲜重、茎鲜重、叶干重和茎干重分别是对照组的1.68倍(P<0.05)、1.88倍(P<0.05)、1.46倍(P>0.05)、3.00倍(P<0.05)、1.68倍(P<0.05)、3.06倍(P<0.05);红、蓝单色光均导致光合色素含量和净光合速率(net photosynthetic rate,P_(n))显著降低,而红蓝复合光促进叶绿素b(chlb)、类胡萝卜素(car)含量和P_(n)的增加,并且随着红光比例的增加,叶绿素含量、car含量、chla/chlb、P_(n)呈显著降低趋势;红蓝单色光和复合光导致气孔导度(stomatal conductance,G_(s))(除RB41处理外)和蒸腾速率(transpiration rate,T_(r))(除RB21和RB41处理外)呈降低趋势。主成分分析结果显示,红蓝1:1复合光(RB11)更利于金秋砂糖橘新梢生长发育、物质积累和光合作用。综上,该研究为建立金秋砂糖橘苗木快速繁育的LED补光技术提供了理论依据。In order to clarify the influence of different light-emitting diode(LED)light quality on growth,development and photosynthetic characteristics of Jinqiu Shatangju,Jinqiu shatangju container seedlings with trifoliate orange as the rootstock were used as the experimental materials,six light quality treatments[red light(R),blue light(B),red and blue composite light(RB11,RB21,RB41,RB81)]were set up in this study with white light(W)as the control.Phenotypic index,biomass,photosynthetic pigment content and photosynthetic characteristic parameters of the newly grown stems and leaves were measured after two months treatment,and the principal component analysis was performed on the relevant indicators.The results showed that red light significantly promoted elongation of new shoots,increased the number of leaves,leaf area and biomass,and inhibited thickening of stems and leaves,while effect of blue light was opposite to that of red light.Low proportion of red light also significantly promoted the elongation of new shoots,made stem thicker,increased number of leaves,leaf area and biomass,however,as the proportion of red light increased,growth trend was significantly reduced.Stem length,stem thickness,number of leaves and leaf area of compound light RB11 were 1.97 times(P<0.05),1.14 times(P>0.05),2.05 times(P<0.05),1.84 times(P<0.05)of the control,respectively.Fresh weight and dry weight of new shoots were 1.68 times(P<0.05),1.88 times(P<0.05)of the control.Fresh weight of leaves and stems,dry weight of leaves and stems were 1.46 times(P>0.05),3.00 times(P<0.05),1.68 times(P<0.05),3.06 times(P<0.05)of the control.Red light and blue light all lead to a significant decrease in photosynthetic pigment content and net photosynthetic rate(P_(n)),while red-blue composite light promoted increase of chlorophyll b(chlb),carotenoid(car)content and P_(n).In addition,increasing the proportion of red light,content of chlorophyll and car,as well as chla/chlb and P_(n) showed significant decreasing trend.All treatment light led to a decrea
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