Genetic Characteristics and Antimicrobial Susceptibility of Arcobacter butzleri Isolates from Raw Chicken Meat and Patients with Diarrhea in China  

作　　者：WANG Yuan Yuan ZHOU Gui Lan LI Ying GU Yi Xin HE Mu ZHANG Shuang JI Guo Qiang YANG Jie WANG Miao MA Hong Mei ZHANG Mao Jun 

机构地区：[1]Microbiology Laboratory,Shunyi District Center for Disease Control and Prevention,Beijing 101399,China [2]State Key Laboratory of Infectious Disease Prevention and Control,Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases,National Institute for Communicable Disease Control and Prevention,Chinese Center for Disease Control and Prevention,Beijing 102206,China

出　　处：《Biomedical and Environmental Sciences》2021年第12期1024-1028,共5页生物医学与环境科学（英文版）

基　　金：supported by National Key Research and Development Program of China [grant No.:2021YFC2301000];the Academic Commission of Shunyi District Center for Disease Control and Prevention,Beijing,China。

摘　　要：Arcobacter is an emerging foodborne pathogen worldwide.In this study,the prevalence,antimicrobial susceptibility and genetic characteristics of Arcobacter from different sources were investigated.Eighteen A.butzleri isolates were obtained from 60 raw chicken meat samples(16/60,27%)and 150 patients with diarrhea(2/150,1.3%).The resistance ratios to nalidixic acid,ciprofloxacin,clindamycin,chloramphenicol,and florfenicol were 83.33%(15/18),38.89%(7/18),38.89%(7/18),33.33%(6/18)and 33.33%(6/18),respectively.We performed whole genome sequencing of the 18 isolates,and we predicted antibiotic resistance genes and virulence factors by using assembled genomes through blastx analysis.Two resistance genes,blaOXA-464 and tet(H),and the C254T mutation in gyrA,were identified in the genomes of some resistant isolates.Furthermore,virulence genes,such as flgG,flhA,flhB,fliI,fliP,motA,cadF,cjl349,ciaB,mviN,pldA and tlyA,were found in all strains,whereas hecA,hecB and iroE were found in only some strains.Phylogenetic tree analysis of A.butzleri isolates on the basis of the core-genome single nucleotide polymorphisms showed that two isolates from patients with diarrhea clustered together,separately from the isolates from raw chicken and the chicken strains.This study is the first comprehensive analysis of Arcobacter isolated in Beijing.

关 键 词：Arcobacter Arcobacter butzleri PREVALENCE Whole genome sequence Antimicrobial susceptibility Genomic structure 

分 类 号：R446.5[医药卫生—诊断学]