薏苡叶斑病病原菌生物学特性及防治药剂毒力评估  

Biological Properties and Fungicide Toxicity on Pathogen of Coix Leaf Spot

作　　者：侯翔宇 黄世勇 姚锦爱[1] 谢世勇[1] 黄建成[1] HOU Xiangyu;HUANG Shiyong;YAO Jinai;XIE Shiyong;HUANG Jiancheng(Fujian Key Laboratory for Monitoring and Integrated Management of Crop Pests/Institute of Plant Protection,Fujian Academy of Agricultural Sciences,Fuzhou,Fujian 350013,China;Seed Station of Wuyishan City,Wuyishan,Fujian 354300,China)

机构地区：[1]福建省作物有害生物监测与治理重点实验室/福建省农业科学院植物保护研究所,福建福州350013 [2]武夷山市种子站,福建武夷山354300

出　　处：《福建农业学报》2021年第12期1464-1470,共7页Fujian Journal of Agricultural Sciences

基　　金：福建省科技计划公益类专项(2018R1025-5);福建省农业科学院项目(CXTD2021016);植物病虫害生物学国家重点实验室项目(SKLOF202125);福建省农业高质量发展超越“5511”创新工程项目(XTCXGC2021011、XTCXGC2021017)。

摘　　要：【目的】明确薏苡叶斑病病原菌生物学特性,评估3类防治药剂对薏苡叶斑病病原菌的防控潜力。【方法】测量薏苡叶斑病病原菌在不同温度、pH、光照条件及碳氮源等培养条件下的菌落直径,测定氯氟醚菌唑、吡唑醚菌酯、咯菌腈等防治药剂的毒力及评估它们二元复配剂的联合作用效果。【结果】薏苡叶斑病病原菌菌丝生长最适温度为25℃,pH为8,最适碳氮源分别为淀粉和蛋白胨,光照对生长的影响不显著;氯氟醚菌唑、吡唑醚菌酯、咯菌腈等3种杀菌剂对薏苡叶斑病病原菌均有很好的抑制作用,毒力指数分别为多菌灵对照的234.83、97.84、84.97倍,它们二元复配剂只有氯氟醚菌唑与吡唑醚菌酯复配有增效作用,配比5∶5时共毒系数达221.85。【结论】温度、pH、碳氮源能显著影响薏苡叶斑病病原菌生长,氯氟醚菌唑、吡唑醚菌酯、咯菌腈等防治药剂对薏苡叶斑病有较好的防控潜力,但它们之间的混用应加以评估。【Objective】Biological properties of Curvularia coicis Castellani,which causes coix leaf spot(CLS)on Chinese pearl barley,and the control efficacy of 3 fungicides on the disease were studied.【Method】Effects of temperature,pH,light,and carbon and nitrogen sources on the mycelial growth of the pathogen as well as the toxicity of mefentrifluconazole,pyraclostrobin,fludioxonil,and their binary compounds toward the pathogen were determined.【Result】The optimum conditions for the mycelial growth of C.coicis were found to be 25℃,pH 8,starch for carbon,and peptone for nitrogen with no specific preference for light exposure.All 3 tested fungicides exhibited inhibitory effects on the pathogenic growth as indicated by the toxicity indices of mefentrifluconazole at 234.83-fold,pyraclostrobin at 97.84-fold,and fludioxonil at 84.97-fold of that of carbendazim.In combination of the agents,only mefentrifluconazole and pyraclostrobin were shown synergistic in the antifungal efficacy with the best mixing ratio of 5∶5 with a toxicity index of 221.85.【Conclusion】Temperature,pH,and carbon and nitrogen sources significantly affected C.coicis growth.Mefentrifluconazole,pyraclostrobin,and fludioxonil were strongly antifungal against the pathogen.But application of combinations of these agents require further evaluation.

关 键 词：薏苡叶斑病 生物学特性 防治药剂 毒力评估 

分 类 号：S436[农业科学—农业昆虫与害虫防治]