PEG400修饰的ZnS:Cu量子点荧光猝灭法检测农药敌草快  

Fluorescence Quenching on PEG400-modified ZnS:Cu Quantum Dots and Herbicide Diquat

作　　者：王艳君 张帆 WANG Yanjun;ZHANG Fan(Fujian Polytechnic Normal University,Fuqing,Fujian 350300,China;Modern Facility Agriculture of Fujian Provincial Higher Education Engineering Research Center,Fuqing,Fujian 350300,China)

机构地区：[1]福建技术师范学院,福建福清350300 [2]现代设施农业福建省高等学校工程研究中心,福建福清350300

出　　处：《福建农业学报》2021年第11期1380-1386,共7页Fujian Journal of Agricultural Sciences

基　　金：国家自然科学基金(21547005);福建省自然科学基金(2019J01893);福建省新世纪优秀人才支持计划(闽教科2016-23);福建省教育厅中青年教师教育科研项目(JAS180651);福建省科技计划项目(2020H4005)。

摘　　要：【目的】为探讨农药敌草快的快速检测方法,采用水热法制备水溶性PEG400修饰的ZnS:Cu量子点,通过荧光猝灭强度进行量子点与敌草快的互作信号表征。【方法】用荧光分光光度计、傅里叶红外光谱仪及紫外-可见分光光度计对合成的复合量子点进行表征,探究PEG400的修饰量对量子点的影响,同时测定量子点对细菌的抑制作用及毒性。【结果】在适宜的反应条件下,敌草快浓度为1.45×10^(−6)~8.7×10^(−6) mol·L^(−1)时,量子点荧光猝灭程度与敌草快的浓度呈现较好的线性关系,检出限为2.071×10−7 mol·L^(−1),相关系数R2达0.9999,是生物相容性好的低毒材料。【结论】所获得的PEG400修饰ZnS:Cu量子点初步可应用于农药敌草快的快速检测中,为相关检测技术的发展奠定基础。【Objective】Water-soluble PEG400-modified ZnS:Cu quantum dots(QDs)were prepared by hydrothermal method in search for an applicable rapid detection method on diquat.【Method】The QDs were characterized by their quenching intensities using a fluorescence spectrophotometer,an FTIR,and a UV-Vis spectrophotometer.Effect of PEG400 on the ZnS:Cu QDs was examined to determine the optimal modification for the establishment of a methodology to rapidly detect the toxicity of diquat on microorganisms.The correlation between QD quenching intensity and diquat concentration was examined for the methodology development.【Result】Under appropriate reaction conditions at the diquat concentration in the range of 1.45×10^(−6)−8.7×10^(−6)mol·L^(−1),the fluorescence quenching degree of QDs had an excellent linear relationship with diquat concentration of a correlation coefficient of 0.9999.The detection limit of the method was 2.071×10−7mol·L^(−1).【Conclusion】The obtained PEG400-modified ZnS:Cu QDs was considered adequate to be applied for rapid detection of diquat in support of further development of related analytical methodology.

关 键 词：ZnS:Cu量子点 PEG400修饰剂 荧光猝灭 敌草快 

分 类 号：S436[农业科学—农业昆虫与害虫防治]