改良法制备自体富血小板血浆及其生长因子含量分析  

A modified method of platelet-rich plasma preparation and level measurement of growth factors

作　　者：薛晨红[1] 黄致蓁 彭芬[1] 陈周[1] 张建中[1] Xue Chenhong;Huang Zhizhen;Peng Fen;Chen Zhou;Zhang Jianzhong(Department of Dermatology,Peking University People's Hospital,Beijing 100044,China;Department of Dermatology,Chang Geng Hospital,Xiamen 361028,China)

机构地区：[1]北京大学人民医院皮肤科,北京100044 [2]厦门长庚医院皮肤科,厦门361028

出　　处：《中华医学美学美容杂志》2021年第6期531-534,共4页Chinese Journal of Medical Aesthetics and Cosmetology

摘　　要：目的建立一个安全、有效、简便、经济的自体富血小板血浆(PRP)的提取方法,并对其中的生长因子含量进行分析。方法2014年1-6月,北京大学人民医院皮肤科就诊的萎缩性痤疮瘢痕患者24例,男10例,女14例,年龄21〜46(28.6±6.0)岁。采集患者肘静脉血用改良的Chouk-roim法制备PRP,采用酶联免疫吸附法(ELISA)定量分析正常血浆及PRP中6种生长因子包括血小板源生长因子(PDGF)-β、转化生长因子(TGF)-β1、表皮生长因子(EGF)、血管内皮生长因子(VEGF)、碱性成纤维细胞生长因子(bFGF)、胰岛素样生长因子(IGF)-1的浓度。结果PRP中PDGF-β浓度(755.61±418.31)ng/L,高于正常血浆(479.93±279.18)ng/L(t=3.479,P<0.01);TGF-β1浓度(2267.00±1223.68)ng/L,高于正常血浆(1535.50±910.91)ng/L.(t=7.082,P<0.01);EGF浓度(30.70±12.39)ng/L,高于正常血浆(20.27±10.31)ng/L,(t=6.899,P<0.01);VEGF浓度(25.42±17.69)ng/L,高于正常血浆(12.01±7.77)ng/L(t=230,P<0.01);VEGF浓度(17.85±7.17)ng/L,高于正常血浆(10.90士4.73)ng/L,(t=6.050,P<0.01);IGF-1浓度(201.22±36.80)ng/ml,高于正常血浆(174.90±33.80)ng/ml(t=3.760,PC0.01)。结论改良法PRP比正常血浆含有更高浓度的生长因子,且制备方法相对简便、经济,所获取的自体液体成分PRP尤其适用于有创的皮肤美容激光术后外涂以促进创面修复。Objective To establish a safe,effective,simple,and more economically feasible method to obtain platelet-rich plasma(PRP).Methods Whole blood was collected from 24 patients with atrophic acne scars on the face.For the preparation of PRP,a slight modification of Choukrouns method was used.Enzyme-linked immunosorbent assay(ELISA)was used to measure the levels of platelet derived growth factor-β(PDGF-β),transforming growth factor-β1(TGF-β1),epidermal growth factor(EGF),vascular endothelial growth factor(VEGF),basic fibroblast growth factor(VEGF),isulin-like growth factor-1(IGF-1)in normal plasma and PRP.Results The average con-centration of PDGF-βin PRP was(755.61±418.31)ng/L,which was higher than[(479.93±279.18)ng/L]in normal plasma(t=3.479,P<O.01).The average concentration of TGF-β1 in PRP was(2267.00±1223.68),which was higher than[(1535.50±910.91)ng/L]in normal plasma(t=7.082,P<0.01).The average concentration of EGF in PRP was(30.70±12.39)ng/L,which was higher than[(20.77±10.31)ng/L]in normal plasma(t=6.899,P<0.01).The average concentration of VEGF in PRP was(25.42±17.69)ng/L,which was higher than[(12.01±7.77)ng/L]in normal plasma (t=5.230,P<0.01).The average concentration of bFGF was(17.85±7.17)ng/L,which was higher than[(10.90±4.73)ng/L]in normal plasma(t=6.050,P<0.01).The average concentration of IGF-1 was(201.22±36.80)ng/ml,which was higher than[(174.90±33.80)ng/ml]in normal plasma(t=3.760,P<0.01).Conclusions Compared to normal plasma,modified PRP contains higher levels of growth factors.The modified method is a reliable option for liquid PRP,especially applicable for invasive cosmetic laser surgery to promote wound repair.

关 键 词：富含血小板血浆 改良法 自体富血小板血浆 生长因子 酶联免疫吸附法 浓度 

分 类 号：R457.14[医药卫生—治疗学]