基于三周期极小曲面β-磷酸三钙仿生骨支架设计和生物活性的检测  

Design and biological activity of beta-tricalcium phosphate biomimetic bone scaffold based on triply periodic minimal surfaces

作　　者：王金斯 王胜法[2] 吴柱国 何晓玲 王馨钰 罗小钰 招轶 张静莹 Wang Jinsi;Wang Shengfa;Wu Zhuguo;He Xiaoling;Wang Xinyu;Luo Xiaoyu;Zhao Yi;Zhang Jingying(Dongguan First Hospital Affiliated to Guangdong Medical University,Dongguan 523808,Guangdong Province,China;DUT-RU International School of Information and Software Engineering,Dalian University of Technology,Dalian 116620,Liaoning Province,China)

机构地区：[1]广东医科大学广东医科大学附属东莞第一医院,广东省东莞市523808 [2]大连理工大学国际信息与软件学院,辽宁省大连市116620

出　　处：《中国组织工程研究》2022年第21期3291-3297,共7页Chinese Journal of Tissue Engineering Research

基　　金：广东省基础与应用基础研究基金联合基金(2020B1515120001),项目负责人:张静莹;广东省普通高校重点领域资助项目(2020ZDZX2013),项目负责人:张静莹;广东医科大学学科建设项目(4SG21019G),项目负责人:张静莹,广东医科大学学科建设项目(4SG21015G),项目负责人:吴柱国。

摘　　要：背景:增材制造技术可以精确、个性化地定制具有复杂多孔结构的骨支架,达到恢复临界骨缺损区域松质骨结构和功能的效果。目的:通过材料学和细胞学表征,明确三周期极小曲面结构β-磷酸三钙生物陶瓷骨支架的机械性能及生物活性,揭示三周期极小曲面结构对成骨细胞的调控效果。方法:通过Matlab R2020a软件设计330,420,510μm 3种孔径的三周期极小曲面G曲面支架,Inspire 2018软件分析支架结构设计。以三周期极小曲面结构导出后的STL文件为蓝本,通过基于数字激光加工的增材制造技术制备3种β-磷酸三钙生物陶瓷支架,采用扫描电镜观测支架表面形貌,X射线衍射仪检测物相组成,万能材料试验机检测支架的力学强度。将MC3T3-E1细胞与3种支架共培养,检测细胞的增殖活性、黏附能力与碱性磷酸酶活性。结果与结论:(1)Inspire 2018软件显示,三周期极小曲面呈现出光滑、连续、均一的贯通式多孔结构;(2)扫描电镜下可见,基于数字激光加工的增材制造技术成功实现了三周期极小曲面结构的精确成型;(3)X射线衍射结果证实,支架由纯β-磷酸三钙晶相组成;(4)3组支架的压缩强度和弹性模量均处于或接近松质骨力学强度范围,且支架的压缩强度与孔径大小呈反比;(5)CCK8实验显示,MC3T3-E1细胞在3种支架上生长良好,生物活性与孔径呈现剂量依赖关系,510μm孔径促进细胞增殖效果最佳;(6)活细胞成像仪和激光共聚焦显微镜下可见,MC3T3-E1细胞能够在3种支架上实现早期黏附,且黏附量随孔径增大而增加;(7)碱性磷酸酶活性分析表明,420μm孔径支架上细胞的碱性磷酸酶活性最高;(8)结果表明,三周期极小曲面结构β-磷酸三钙生物陶瓷支架表现出较好的机械性能与生物活性,其中420μm孔径有利于促进细胞分化,510μm孔径有利于细胞增殖,具有修复临界骨缺损的应用潜力。BACKGROUND:The bone scaffold with complex porous structure can be customized accurately and individually by additive manufacturing technology,so as to achieve the dual bionics of the structure and function of cancellous bone at critical-sized bone defects.OBJECTIVE:To clear the mechanical properties and biological activity ofβ-tricalcium phosphate bioceramic bone scaffold with triply periodic minimal surface structure,and reveal the regulatory effect of triply periodic minimal surface structure on osteoblasts through material and cytological characterizations.METHODS:Three kinds of triply periodic minimal surface G surface structureβ-tricalcium phosphate bioceramic bone scaffolds with different pore sizes of 330,420,and 510μm were designed by Matlab R2020 a software,and the design drawings were analyzed by Inspire 2018 software.The STL file exported based on the triply periodic minimal surface structure was the blueprint.β-Tricalcium phosphate scaffolds were fabricated by additive manufacturing technology based on digital laser processing.The surface morphology was observed by scanning electron microscope.The phase composition was detected by X-ray diffractometer,and the mechanical strength was detected by universal material testing machine.MC3 T3-E1 cells were co-cultured with scaffolds.Cell proliferation,cell adhesion,and alkaline phosphatase activity were detected.RESULTS AND CONCLUSION:(1)Inspire 2018 software showed that triply periodic minimal surface presented a smooth,continuous and uniform porous structure.(2)Scanning electron microscope confirmed that the additive manufacturing technology based on digital laser processing successfully realized the accurate molding of triply periodic minimal surface structure.(3)X-ray diffraction confirmed that the scaffold was composed of pureβ-tricalcium phosphate crystal phase.(4)The compressive strength and elastic modulus of the three groups of scaffolds were all within or near the range of cancellous bone,and the compressive strength of the scaffolds was inver

关 键 词：三周期极小曲面 生物陶瓷 Β-磷酸三钙 增材制造 数字激光加工 临界骨缺损 孔径 MC3T3-E1细胞 

分 类 号：R459.9[医药卫生—治疗学] R318.08[医药卫生—临床医学]