壳聚糖改性聚醚醚酮表征及对MC3T3-E1细胞黏附、增殖的影响  

Characterization of chitosan-modified polyetheretherketone and its effect on MC3T3-E1 cell adhesion and proliferation

作　　者：冯乐 邱鹏 刘敏 周会 Feng Le;Qiu Peng;Liu Min;Zhou Hui(Oral and Maxillofacial Reconstruction and Regeneration Laboratory,Southwest Medical University,Department of Prosthodontics,Affiliated Stomatological Hospital,Southwest Medical University,Luzhou 646000,Sichuan Province,China)

机构地区：[1]西南医科大学口颌面修复重建与再生实验室西南医科大学附属口腔医院修复科,四川省泸州市646000

出　　处：《中国组织工程研究》2022年第21期3351-3356,共6页Chinese Journal of Tissue Engineering Research

基　　金：四川省医学科研课题计划项目(S19023),项目负责人:刘敏;泸州市科技计划项目(2018-JYJ-39),项目负责人:周会。

摘　　要：背景:聚醚醚酮的生物惰性表面限制了其医学应用,如何提高聚醚醚酮的生物活性亟待解决。目的:分析壳聚糖生物活性涂层改性聚醚醚酮的表面特征及其对MC3T3-E1细胞增殖、黏附的影响。方法:取圆片状聚醚醚酮材料,依次进行NaBH4、3-氨丙基三乙氧基硅烷、戊二醛水溶液及壳聚糖溶液处理,获得壳聚糖生物活性涂层改性的聚醚醚酮材料。使用X射线光电子能谱、扫描电镜、原子力显微镜与全自动接触角测量仪观察化学处理前后聚醚醚酮材料的表面特征。将MC3T3-E1细胞分别接种于聚醚醚酮与壳聚糖改性聚醚醚酮材料表面,观察细胞的增殖与黏附情况。结果与结论:(1)X射线光电子能谱检测显示,聚醚醚酮材料仅含有C、O元素,壳聚糖改性聚醚醚酮材料含有C、O、N、Si元素;壳聚糖改性聚醚醚酮材料表面的接触角小于聚醚醚酮材料(P<0.05);(2)扫描电镜下可见,聚醚醚酮材料表面有明显的凹槽状起伏,壳聚糖改性聚醚醚酮材料表面存在壳聚糖分子,大小为1.0-2.0μm;原子力显微镜下可见,聚醚醚酮材料表面有较多的微小凹坑,大小约为0.1μm,壳聚糖改性聚醚醚酮材料表面的凹坑增大,大小为0.2-0.5μm,表面粗糙度大于聚醚醚酮材料;(3)倒置显微镜下可见,壳聚糖改性聚醚醚酮材料表面的细胞数量多于聚醚醚酮材料(P<0.05);激光共聚焦显微镜下可见,黏附在聚醚醚酮材料表面的细胞伸展性较差、伪足较少、肌动蛋白微丝不明显,黏附在壳聚糖改性聚醚醚酮材料表面的细胞伸展性较好、伪足较多、肌动蛋白微丝多且明显;(4)CCK-8实验显示,壳聚糖改性聚醚醚酮材料表面的细胞增殖快于聚醚醚酮材料(P<0.05);(5)结果表明,壳聚糖表面改性增加了聚醚醚酮材料表面的粗糙度和湿润性,促进材料表面MC3T3-E1细胞的增殖、黏附。BACKGROUND:The biological inert surface of polyetheretherketone limits its medical application,so how to improve the biological activity of polyetheretherketone needs to be solved urgently.OBJECTIVE:To analyze the surface characteristics of polyetheretherketone modified by chitosan bioactive coating and its effect on the adhesion and proliferation of MC3 T3-E1 cells.METHODS:Disc-shaped polyetheretherketone material was obtained and treated with Na BH4,3-aminopropyltriethoxysilane,glutaraldehyde aqueous solution,and chitosan solution to form polyetheretherketone modified with chitosan bioactive coating.X-ray photoelectron spectroscopy,scanning electron microscope,atomic force microscope,and automatic contact angle measurement instrument were used to observe the surface characteristics of chemically treated polyetheretherketone.MC3 T3-E1 cells were seeded on the surface of polyetheretherketone and chitosan-modified polyetheretherketone separately,and the proliferation and adhesion of the cells were observed.RESULTS AND CONCLUSION:(1)X-ray photoelectron spectroscopy showed that the polyetheretherketone material only contained C and O elements.The chitosan modified polyetheretherketone material contained C,O,N,and Si elements.The contact angle on the surface of chitosan modified polyetheretherketone material was smaller than that of the polyetheretherketone material(P<0.05).(2)Under the scanning electron microscope,the surface of the polyetheretherketone material had obvious groove-like undulations,and the surface of the chitosan-modified polyetheretherketone material had chitosan molecule.The size was 1.0-2.0μm.Atomic force microscope exhibited that there were many tiny pits on the surface of the polyetheretherketone material,and the size was about 0.1μm.The pits on the surface of the chitosan-modified polyetheretherketone material increased;the size was 0.2-0.5μm,and the surface roughness was greater than that of the polyetheretherketone material.(3)Under an inverted microscope,the number of cells on the surface

关 键 词：聚醚醚酮 壳聚糖 生物材料 表面改性 生物安全性 成骨细胞 种植体 细胞增殖 细胞黏附 

分 类 号：R459.9[医药卫生—治疗学] R318.08[医药卫生—临床医学]